Tn5转座突变技术在革兰氏阴性细菌分子遗传研究中的应用

中国生物工程杂志

2009, Vol. 29

Issue (12): 114-118

综述

Tn5转座突变技术在革兰氏阴性细菌分子遗传研究中的应用

年洪娟^**,陈丽梅,李昆志

昆明理工大学生物工程技术研究中心昆明 650224

Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gramnegative Bacteria

NIAN Hong-juan,CHEN Li-mei,LI Kun-zhi

Biotechnology Research Center, Kunming University of Science and Technology,Kunming 650224,China

全文: PDF(444 KB) HTML

摘要：

随着广宿主载体系统的发展，Tn5及其衍生载体已经广泛应用于革兰氏阴性细菌的分子遗传学研究。主要综述了Tn5转座突变技术在生防细菌生防机理研究、细菌必需基因的鉴定、病原细菌毒力相关基因研究、代谢调控基因研究和菌株的遗传改良方面的应用研究进展。

关键词： Tn5转座突变; 生防机理; 必需基因; 细菌毒力基因; 代谢调控基因; 菌株遗传改良

Abstract:

With development of wide-host-range vector systems, Tn5 transposon and its derivative vectors have been widely applied to genetic research of gram-negative bacteria.The applications of Tn5 transposon mutagenesis technology to genetic researches of bacteria were briefly discussed, including researches on biological control mechanisms of biocontrol bacteria, identification of bacterial essential genes, discovering virulence genes of bacterial pathogens, characterization of metabolism regulatory genes and genetic improvements of bacteria.

Key words: Tn5 transposon mutagenesis Biocontrol mechanism Bacterial essential geneVirulence gene Metabolism regulatory gene Bacteria improvements

收稿日期: 2009-05-06 出版日期: 2009-12-21

ZTFLH:

Q819

基金资助:

云南省应用基础研究基金(2009ZCO14X)、云南省教育厅科学研究基金(09C0106)资助项目

通讯作者: 年洪娟 E-mail: hjnian@163.com

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引用本文:

年洪娟陈丽梅李昆志. Tn5转座突变技术在革兰氏阴性细菌分子遗传研究中的应用[J]. 中国生物工程杂志, 2009, 29(12): 114-118.

NIAN Hong-Juan, CHEN Li-Mei, LI Hun-Zhi. Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gramnegative Bacteria. China Biotechnology, 2009, 29(12): 114-118.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I12/114

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