链球菌G蛋白IgG结合域(GBx)的融合表达及其IgG结合活性的比较分析

黄雪年 许杨 李燕萍

中国生物工程杂志 ›› 2009, Vol. 29 ›› Issue (10) : 64-68.

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PDF(525 KB)
中国生物工程杂志 ›› 2009, Vol. 29 ›› Issue (10) : 64-68.
研究报告

链球菌G蛋白IgG结合域(GBx)的融合表达及其IgG结合活性的比较分析

  • 黄雪年,许杨*,李燕萍
作者信息 +

Study on Fused Expression of the IgG-binding Domain of Streptococcal Protein G and Its IgG-binding Activity

  • HUANG Xue-nian,XU Yang,LI Yan-ping
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文章历史 +

摘要

链球菌G蛋白的IgG结合域能够特异性地结合IgG 的Fc区,是制备免疫微阵列的一种理想的IgG固定材料。克隆表达了具有IgG结合活性的3种IgG结合域的GST融合蛋白(GST-GBx),该3种蛋白分别含有1个、2个和3个IgG结合域。采用ELISA对三蛋白IgG结合能力进行了比较分析。结果表明在含B-Domain的量相同的情况下,GST-GB3蛋白固定IgG的量最多,其次为GST-GB2,GST-GB1最弱;对IgG的灵敏度也是GST-GB3最强,GST-GB1最弱,提示GST-GB3固定IgG的能力较其他两蛋白具有明显优势。

Abstract

The IgG binding domain of Streptococcal Protein G which can selectively immobilizes the Fc regions of immunoglobulin G (IgG) is a kind of good material for oriented immobilization of antibodies in antibody microarrays. Here, genetically engineered three glutathione S-transferase (GST) fused proteins ,bearing one, two and three B-Domains respectively(GST-GBx). The IgG-bindding ability of GST-GBx was investigated by ELISA. The date revealed that when the B-domain’s quantity of GST-GBx is identical, the GST-GB3 is the most efficient protein among three GST-GBx protein both the capacity and sensibility of binding IgG. The GST-GB2 is the next one and GST-GB1 is the least one. Thus,the GST-GB3 has significantly predominance in comparison to GST-GB2 and GST-GB1.

关键词

链球菌G蛋白 / IgG结合域 / GST融合蛋白 / IgG结合能力 / ELISA

Key words

Streptococcal Protein G / B-domain / GST fused proteins / IgG-binding ability / ELISA

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导出引用
黄雪年 许杨 李燕萍. 链球菌G蛋白IgG结合域(GBx)的融合表达及其IgG结合活性的比较分析[J]. 中国生物工程杂志, 2009, 29(10): 64-68
HUANG Xue-Nian, HU Yang, LI Yan-Ping. Study on Fused Expression of the IgG-binding Domain of Streptococcal Protein G and Its IgG-binding Activity[J]. China Biotechnology, 2009, 29(10): 64-68
中图分类号: Q819   

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