p300通过homeobox结构域增强Nanog的转录激活活性

中国生物工程杂志

2009, Vol. 29

Issue (10): 1-5

研究报告

p300通过homeobox结构域增强Nanog的转录激活活性

郭允倩¹,陈默²,马天骅²,裴端卿³

1. 清华大学医学院生物膜与膜生物工程国家重点实验室北京 100084
2.中国科学院广州生物医药与健康研究院再生生物学国家重点实验室广州 510663
3. 北京林业大学林木育种国家工程实验室林木、花卉遗传育种教育部重点实验室北京 100083

P300 Enhances Nanog Transcriptional Activity Through the Homeobox Domain

1.State Key Laboratory of Biomembrane and Membrane Biotechnology,Institutes of Biomedicine,School of Medicine,Tsinghua University,Beijing 100084,China
2.Key Laboratory of Regenerative Biology,Guangzhou Institute of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510663,China
3.National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beiming Forestry University,Beijing 100083,China.

全文: PDF(1233 KB) HTML

摘要：

胚胎干细胞(ES细胞)能够不断地进行自我更新来维持其多能性，很多转录因子共同调控着ES细胞的自我更新和多能性，Nanog就是其中之一，然而Nanog维持ES细胞多能性的机制并不清楚。p300是真核生物中普遍存在的转录辅助因子，与许多转录因子共同作用调控下游基因的表达。为探索p300是不是能够影响Nanog的转录活性，我们在细胞中共转Nanog(或突变体)及报告基因和p300，结果表明p300能够通过homeobox结构域增强Nanog的转录激活活性。

关键词： ES细胞; Nanog; p300; homeobox结构域; 转录活性

Abstract:

Embryonic stem cells (ES cells) undergo selfrenewal to maintain their pluripotency. The selfrenewal and pluripotency of ES cells are under tight control of many transcription factors. Nanog is one of these transcription factors. However, the molecular mechanisms under which Nanog regulates ES cell pluripotency are not very clear. P300 is a general coactivator in eukaryotes which usually helps transcription factors to regulate downstream genes. Nanog (or Nanog mutants) and its reporter gene were cotransfeted with p300 in cells in order to see if p300 could affect Nanog transcriptional activity. The results showed that p300 enhances Nanog transcriptional activity through the homeobox domain.

Key words: ES cell Nanog p300 homeobox domain transcriptional activity

收稿日期: 2009-05-05 出版日期: 2009-10-29

ZTFLH:

Q756

基金资助:

国家自然科学基金 (30725012, 30630039，90813033)、中国科学院知识创新工程(KSCX2YWR48)资助项目

通讯作者: 郭允倩 E-mail: pei_duanqing@gibh.ac.cn

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引用本文:

郭允倩陈默马天骅裴端卿. p300通过homeobox结构域增强Nanog的转录激活活性[J]. 中国生物工程杂志, 2009, 29(10): 1-5.

GUO Yuan-Qian, CHEN Mo, MA Tian-Hua, FEI Duan-Qing. P300 Enhances Nanog Transcriptional Activity Through the Homeobox Domain. China Biotechnology, 2009, 29(10): 1-5.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I10/1

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