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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2009, Vol. 29 Issue (03): 41-46    
研究报告     
γ-聚谷氨酸与D-半乳糖酯化衍生物-顺铂复合物的制备及其生物活性
曹小红,闫乐,王春玲,焦润芝,鲁梅芳
天津科技大学食品工程与生物技术学院
Preparation and Biological Activity of Poly (γ-glutamic acid)-D-galactose- Cisplatin Complex Compound
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摘要:

介绍了制备一种γ-聚谷氨酸-D-半乳糖酯化衍生物-顺铂复合物[poly (γ-glutamic acid)-D-galactose esterifiable derivative-cisplatin complex compound,γ-D+-DDP],并考察其抗肿瘤活性。主要通过生物发酵获得大分子γ-聚谷氨酸[poly (γ-glutamic acid) ,γ-PGA],利用酸降解得到可以作为药物载体的小分子γ-聚谷氨酸;利用凝胶色谱柱检验小分子γ-聚谷氨酸的分子量;利用MTT法检测该复合物的体外抗肿瘤作用。结果表明:成功获得γ-聚谷氨酸-D-半乳糖酯化衍生物-顺铂复合物,该复合物载药率达9.4% ~10.2%;该复合物对人肝癌细胞BEL-7402具有显著的杀伤作用,能引起细胞凋亡(HE染色观察得到)。因此,γ-聚谷氨酸-D-半乳糖酯化衍生物-顺铂复合物是一种有效的抗肿瘤药物,具有潜在的临床应用价值;生物发酵的γ-聚谷氨酸可用于药物载体,赋予药物新的特点。

关键词: 肝靶向性给药系统;γ-聚谷氨酸;顺铂;毒性;抗肿瘤活性    
Abstract:

In order to express and purify the mono-phosphorylated PZR proteins, the bi-phosphorylated-site His-PZR recombined prokaryotic expressions plasmids was used as the template, then through the overlapping PCR the mono- phosphorylated-site PZR-Phe263(TTT) and PZR-Phe241(TTT) mutants were amplified. After recombined and purified, the mutants' and C-Src recombined prokaryotic expressions plasmids were co-transformed into the E.Coli. BL21. Through IPTG induction and Ni-NTA purification, the mono-phosphorylated PZR a and PZR b proteins were obtained successfully. These results laid a foundation for future study on the PZR bio-function mechanism.

Key words: Targeted drug delivery;Poly(γ-glutamic acid);Cisplatin;Toxicity;Antitumor efficiency
收稿日期: 2008-10-10 出版日期: 2009-03-31
基金资助:

国家“863”计划资助项目

通讯作者: 曹小红   
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曹小红 闫乐 王春玲 焦润芝 鲁梅芳

引用本文:

曹小红,闫乐,王春玲,焦润芝,鲁梅芳. γ-聚谷氨酸与D-半乳糖酯化衍生物-顺铂复合物的制备及其生物活性[J]. 中国生物工程杂志, 2009, 29(03): 41-46.

CAO Xiao-Gong- Yan-Le- Wang-Chun-Ling- Jiao-Run-Zhi- Lu-Mei-Fang. Preparation and Biological Activity of Poly (γ-glutamic acid)-D-galactose- Cisplatin Complex Compound. China Biotechnology, 2009, 29(03): 41-46.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I03/41

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