为了研究表达猪2型圆环病毒ORF2基因和猪细小病毒VP2基因的重组伪狂犬病毒疫苗株SA215(D)的疫苗价值，对该病毒株安全性、免疫原性、保护力及对PRV Fa株定值进行了研究。研究结果表明重组病毒SA215（D）毒力显著低于PRV Fa强毒株，可以抵御伪狂犬病强毒Fa株的攻击，并能抵御其在小鼠体内的定植；抗体检测显示SA215（D）免疫小鼠后第2周抗PRV、PCV2和PPV的抗体水平均很低，加强免疫后第4周抗体水平大幅上升，且显著高于阴性对照组，而与阳性对照组无明显差异，表明SA215（D）可诱导小鼠产生体液免疫反应。淋巴细胞增殖试验显示SA215（D）可诱导小鼠产生较强的淋巴细胞增殖反应，与亲本株SA215组相比，差异显著(0.01
The recombinant pseudorabies virus expressing ORF2 gene of porcine circovius type 2 and VP2 gene of porcine parvovirus was developed into trivalence genetically engineering vaccine to inject mice, and the result showed that the recombinant virus SA215（D）had lower virulence than velogenic strain which could resist the attack of PRV Fa strain and successfully induced humoral and cellular immune response in mice. This study established the foundation for the development of trivalence genetically engineering vaccine .
王新,郭万柱,周婷. 猪圆环病毒2型－细小病毒－伪狂犬重组病毒免疫小鼠试验研究[J]. 中国生物工程杂志, 2008, 28(7): 43-47.
. Study on the immunogenicity of recombinant virus of pseudorabies- porcine Circovius Type 2-porcine parvovirus. China Biotechnology, 2008, 28(7): 43-47.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I7/43
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