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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2024, Vol. 44 Issue (1): 118-127    DOI: 10.13523/j.cb.2307024
综述     
微生物群体感应在废水生物处理中的应用*
王倩1,2,**(),覃溢璇1,孔强1,2,李慧宇1,宗可金1,王颖慧1,荣明慧1
1 山东师范大学地理与环境学院 济南 250358
2 山东师范大学东营研究院 东营 257343
Research Progress of Microbial Quorum Sensing in Wastewater Biological Treatment
Qian WANG1,2,**(),Yixuan QIN1,Qiang KONG1,2,Huiyu LI1,Kejin ZONG1,Yinghui WANG1,Minghui RONG1
1 School of Geography and Environment of Shandong Normal University, Jinan 250358, China
2 Dongying Research Institute of Shandong Normal University, Dongying 257343, China
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摘要：

群体感应(quorum sensing,QS)作为依赖菌群密度调控基因表达的基因调控系统,被称为细胞间的交流语言。QS在许多自然及废水处理工程系统中被发现,对环境修复及废水生物处理资源化起到重要的促进作用。总结细菌三种类型的典型信号分子(酰基高丝氨酸内酯类、寡肽类和呋喃酰硼酸二酯类)介导的QS机理,综述信号分子的添加或猝灭、基因的遗传操作、组学技术这三种研究QS系统功能的技术及并对比分析各种方法的优缺点,探讨QS在工业废水生物处理领域中活性污泥、生物膜及生物脱氮方面的应用。结合目前对QS的跨界通讯在废水处理方面的应用潜力和已知研究内容的局限性,对QS系统介导的细菌-植物、细菌-噬菌体、细菌-真菌跨界通讯机制等方面的研究进行展望,以期为利用QS调控策略强化废水处理效率提供新的思路。
关键词： 废水生物处理;  细菌;  群体感应;  信号分子    
Abstract:

Quorum sensing(QS), known as the language of intercellular communication is a gene regulatory system that relies on colony density. It is found in many natural and wastewater treatment engineering systems and plays an important role in environmental remediation and wastewater biotreatment.At present,there is a lack of systematic summary of the research methods and related applications of QS regulating bacterial function.In this paper,the QS mechanism mediated by three types of typical signal molecules(N-Acyl homoserine 1actones,autoinducing peptides and furanosyl borate diEnvironmental Science & Technologyer)of bacteria was summarized.The three techniques of adding or quenching signal molecules,genetic manipulation of genes,and omics techniques to study the function of the QS system were reviewed,and their advantages and disadvantages were compared and analyzed.The application of QS in activated sludge,biofilm and biological nitrogen removal in the field of industrial wastewater biological treatment was discussed.Finally,combined with the current application potential of QS cross-border communication in wastewater treatment and the limitations of known research contents,some studies on QS system-mediated bacterial-plant,bacterial-phage,bacterial-fungal cross-border communication mechanisms were discussed,in order to provide new ideas for using QS regulation strategies to enhance wastewater treatment efficiency in the future.
Key words: Wastewater biological treatment    Bacteria    Quorum sensing    Signal molecule
收稿日期: 2023-07-17 出版日期: 2024-02-04
ZTFLH:  X703  
基金资助: *山东省自然科学基金(ZR2020YQ41)
通讯作者: ** 电子信箱:qianwang86@sdnu.edu.cn   
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王倩
覃溢璇
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李慧宇
宗可金
王颖慧
荣明慧

引用本文:

王倩, 覃溢璇, 孔强, 李慧宇, 宗可金, 王颖慧, 荣明慧. 微生物群体感应在废水生物处理中的应用*[J]. 中国生物工程杂志, 2024, 44(1): 118-127.

Qian WANG, Yixuan QIN, Qiang KONG, Huiyu LI, Kejin ZONG, Yinghui WANG, Minghui RONG. Research Progress of Microbial Quorum Sensing in Wastewater Biological Treatment. China Biotechnology, 2024, 44(1): 118-127.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2307024    或    https://manu60.magtech.com.cn/biotech/CN/Y2024/V44/I1/118

图1  AHL分子结构式
图2  典型的AHL-QS系统(A)与AIP-QS系统(B)
图3  哈氏弧菌中AI-1与AI-2介导的QS系统 图中Р表示信号通过磷酸化传递,H和D分别表示组氨酸和天冬氨酸,为信号传递蛋白的磷酸化残基
应用领域 外源添加物名称 外源添加
物类型		 QS的促
进(+)
/抑制
(-)		 应用效果 参考文献
活性污泥 香草醛(vanillin) QSI - 香草醛减弱QS-AHL系统,延缓或抑制EPS中蛋白(PN)的产生,显著抑制丝状膨胀 [51]
C12-HSL、C14-HSL AHL + 抑制青霉菌菌丝体形成,缓解真菌性污泥膨胀,沉降能力分别提高了6.1%(C12-HSL)和39.7%(C14-HSL) [52]
C6-HSL AHL + 促进AGS快速粒化,提高AGS系统稳定性以及脱氮除磷性能 [55]
AGS中分离的
AHLs上清液		 QS菌 + AGS沉降性能优异(SVI10 37.2 mL/g),加速成粒(颗粒完整性系数为4.4%) [56]
生物膜 C6-HSL、3OC12-HSL AHL + 提高BESs电子摄取量(1.3~2.0倍)和反硝化速率(大于1.0倍),加速阴极生物膜的形成和启动 [57]
D-酪氨酸 QSI - 减少22%生物附着量,抑制活性污泥生物膜污染 [58]
Rhodococcus sp. BH4 QQ菌 - 减少MFC阳极微生物EPS的产生,缓解膜污染,维持良好电化学活性(1 924 mW/m2) [59]
Acinetobacter、Comamonas、
Stenotrophomonas		 QQ菌 - 大幅缓解AnMBR膜污染,将膜过滤时间延长3.72倍(P≤ 0.05) [60]
酰化酶 QQ酶 - 生物膜的黏附强度至少降低37%,生物膜厚度由1 562 μm减少到1 765 μm [61]
生物脱氮 C12-HSL AHL + 明显强化好氧氨氧化过程,导致亚氮积累 [62]
C12-HSL AHL + 显著增加氧限制自养硝化/反硝化生物膜中的厌氧氨氧化速率(P< 0.05) [63]
C14-HSL、3-oxo-C14-HSL、
C6-HSL、3-oxo-C6-HSL		 AHL + 处理16 d后,活性污泥氨氧化率提高了73% [64]
3-oxo-C6-HSL、
C6-HSL、C8-HSL		 AHL + 显著提高SBR(P< 0.05)第二阶段运行期间的脱氮率(NRR) [50]
硼 活化剂 + 刺激微生物自分泌AI-2,促进AOB并抑制NOB,造成N O 2 -累积,促进厌氧氨氧化,进而提高脱氮效率 [67]
蛭形轮虫 微型动物 + 分泌AHLs信号分子类似物,触发QS提高活性污泥对COD、氨氮、总磷的去除率 [68]
表1  群体感应在废水生物处理中的应用
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