中国生物工程杂志

Objective: To prepare the exosomes that display the vesicular stomatitis virus glycoprotein (VSVG) by utilizing genetic engineering technology, which promotes membrane fusion. Meanwhile, the exosomes were further modified with aptamers that target dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) receptors to prepare functionalized exosomes, which is achieved through DNA hybridization chain reaction mediated by nucleic acid probes. This approach reprograms the targeted recognition process between tumor cells and dendritic cells, ultimately enhancing their interaction. Methods: The paper focuses on mouse mammary tumor cells (4T1) and mouse dendritic cells (DC2.4) as research subjects, which demonstrates through confocal imaging and flow cytometry that VSVG-Exos can specifically bind to 4T1 cells via membrane fusion, and that DC-SIGN aptamers can specifically target DC2.4 cells. Results: Functionalized exosomes can reprogram and modify 4T1 cells to enhance their targeted recognition effect with DC2.4 cells. Conclusion: Functionalized exosomes can effectively deliver molecules with specific functions to the surface of tumor cells and reprogram and modify them, thus improving the ability of immune cells to accurately locate and efficiently attack tumor cells. This provides a new approach and strategy for targeted elimination of tumor cells.