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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2022, Vol. 42 Issue (12): 79-90    DOI: 10.13523/j.cb.2207014
综述     
香豆素类化合物功能及生物合成研究进展*
王荣香1,2,宋佳2,孙博2,闫雪3,张万忠1,**(),赵晨2,**()
1 沈阳化工大学制药与生物工程学院 沈阳 110142
2 国家粮食和物资储备局科学研究院 粮食储运国家工程研究中心 北京 100037
3 新希望六和股份有限公司 畜禽饲料与畜禽产品质量安全控制四川省重点实验室 成都 610023
Research Progress of Function and Biosynthesis of Coumarins
WANG Rong-xiang1,2,SONG Jia2,SUN Bo2,YAN Xue3,ZHANG Wan-zhong1,**(),ZHAO Chen2,**()
1 College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China
2 National Engineering Research Center of Grain Storage and Logistics, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China
3 Key Laboratory of Quality Control for Feed and Products of Livestock and Poultry of Sichuan Province, New Hope Liuhe Limited Company, Chengdu 610023, China
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摘要：

香豆素类化合物是自然界中一类重要的化合物,具有抗肿瘤、抗凝血、抗菌、杀虫等多种生物活性,应用领域广泛。目前大多数香豆素类化合物从植物中提取,受环境因素影响较大,得率低、成本高,不利于大规模生产,从而限制了其应用和推广。利用合成生物学的思路合成香豆素类化合物具有无污染、原料易得、成本低、过程可控等优势。对香豆素类化合物生物合成途径的研究,尤其是靶标天然产物生物合成表达元件、宿主和发酵条件的优化,以及合成途径中关键酶的挖掘,已经成为研究热点。综述香豆素类化合物及其衍生物的结构、功能和生物合成研究进展,为其生物合成路径中的基因挖掘及异源表达提供参考。
关键词： 香豆素类化合物;  生物合成;  生物活性;  异源表达    
Abstract:

Coumarins are important compounds in nature. They show a wide range of applications, due to their multiple bio-activities such as antitumor, anticoagulation, antibacterial, and insecticidal. At present, most of these compounds are obtained through plant extraction, which is greatly affected by environmental factors, resulting in low yield and high cost, and therefore is not conducive to large-scale production and hinders their application. Instead, the biosynthesis process is controllable. It becomes a research hotspot to develop industrialized production technology of target natural products through optimization of the biosynthetic expression elements, host and fermentation conditions. However, mining of key enzymes in the biosynthetic pathway is still a difficult task in this research field. In this paper, the structure, function and biosynthesis of some coumarins and their derivatives are reviewed, which provides a reference for gene mining and heterologous expression of the synthetic pathways of these compounds.
Key words: Coumarin compounds    Biosynthesis    Bio-activity    Heterologous expression
收稿日期: 2022-07-08 出版日期: 2023-01-05
ZTFLH:  Q819  
基金资助: *中央级公益性科研院所基本科研业务费专项资助项目(JY2102);中央级公益性科研院所基本科研业务费专项资助项目(ZX2004)
通讯作者: 张万忠,赵晨     E-mail: lzwz2004@sina.com;zc@ags.ac.cn
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引用本文:

王荣香,宋佳,孙博,闫雪,张万忠,赵晨. 香豆素类化合物功能及生物合成研究进展*[J]. 中国生物工程杂志, 2022, 42(12): 79-90.

WANG Rong-xiang,SONG Jia,SUN Bo,YAN Xue,ZHANG Wan-zhong,ZHAO Chen. Research Progress of Function and Biosynthesis of Coumarins. China Biotechnology, 2022, 42(12): 79-90.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2207014    或    https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I12/79

图1  香豆素类化合物的类型及代表化合物
名称 结构 主要功能 参考文献
欧前胡素(imperatorin) 抗炎、抗菌、抗病毒、抗癌、逆转癌细胞耐药性、治疗心血管及神经系统疾病,等 [5]
东莨菪素(scopoletin) 抗癌、抗炎、镇痛、降血压、降血脂,等 [6]
花椒毒素(zanthoxylin) 抗氧化,等 [7]
蛇床子素(osthol) 抗炎止痛、抗菌止痒、抗氧化和神经保护,等 [8]
异补骨脂素(angelicin) 抗糖尿病、抗癌、抗病毒,等 [9]
佛手柑内酯(bergamot lactone) 抗肿瘤、调节血糖、改善失眠、防治骨质疏松、抗衰老、抗炎、抗过敏,等 [10]
七叶内酯(esculetin) 抗肿瘤、抗乙型肝炎病毒 [11]
艾芦司他(irosustat) 抗肿瘤(已进入临床试验阶段) [12]
7-羟基香豆素(7-hydroxycoumarin) 荧光化合物、防晒剂、抑菌 [13]
香豆素A9(coumarin A9) 抗横纹病毒 [14]
补骨脂定(psoralidin) 抗骨质疏松 [15]
华法林(warfarin) 香豆素类口服抗凝药 [16]
4-羟基香豆素(4-hydroxycoumarin) 亲电和亲核性质,抗凝血、抗菌、抗病毒、抗肿瘤,等 [17]
表1  常见香豆素类化合物及其衍生物的结构与功能
图2  香豆素类化合物生物合成途径
图3  莽草酸途径
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