细胞表面展示技术在环境修复方面的研究进展<sup>*</sup>

doi:10.13523/j.cb.2205046

中国生物工程杂志

2022, Vol. 42

Issue (9): 105-115 DOI: 10.13523/j.cb.2205046

综述

细胞表面展示技术在环境修复方面的研究进展^*

黄明珠^1,²,姚坤²,宋卓琳²,张豪²,刘斌²,陈雪岚^1,^2,^**(

)

1.国家淡水鱼加工技术研发专业中心南昌 330022
2.江西师范大学生命科学学院南昌 330022

Advances in Cell Surface Display Technology in Environmental Remediation

HUANG Ming-zhu^1,²,YAO Kun²,SONG Zhuo-lin²,ZHANG Hao²,LIU Bin²,CHEN Xue-lan^1,^2,^**(

)

1. National R&D Center for Freshwater Fish Processing, Nanchang 330022, China
2. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China

全文: PDF(1297 KB) HTML

摘要：

人类社会工业化导致各种有毒物质被排放到环境中造成严重的污染。除了自然降解外,传统的处理方法包括化学转化、物理吸附、离子交换和电化学方法等,但存在二次污染、能源需求高、投资成本高、再生效率低、低浓度废水处理效率低等缺点。细胞表面展示技术是一种通过表面锚定蛋白在细胞表面连接功能肽的新型、高效的生物技术。与细胞内和分泌物表达系统相比,微生物表面展示的蛋白质对有机溶剂、蛋白酶、温度和pH的变化表现出更强的稳定性。通过细胞培养就可以获得固定在细胞表面的蛋白酶,避免了蛋白质纯化、浓缩等繁琐的程序。此外,细胞表面展示技术是良好的单细胞水平突变体文库高通量筛选平台。综述细胞表面展示技术在环境生物修复方面的研究进展,重点介绍该技术的应用和未来发展前景。

关键词： 生物修复; 生物吸附; 细胞表面展示; 全细胞催化; 微生物

Abstract:

Industrialization has led to various toxic compounds emission, which causes environmental contamination. Aside from natural geological weathering, traditional solutions include chemical conversion, physical absorption, electrochemical methods and ions exchange, but their applications are limited due to some disadvantages, such as secondary pollution, high energy requirement, high investment cost, low regeneration efficiency and inefficiency in low-concentration wastewater treatment. Cell surface engineering is an innovative, cost-effective biotechnology of microorganism for modification of cell surface function through joining external functional peptides with surface anchoring proteins. In contrast to conventional intracellular and secretion expression systems, proteins displayed on the surface of microorganism may exhibit enhanced stability against changes in organic solvents, proteases, temperature and pH. Surface-engineered cells prepared by cultivation are ready to be used as microparticles covered with proteins/peptides, avoiding troublesome concentration procedures and protein purification. Furthermore, cell-surface display engineering is suitable for high throughput screening from the mutant library for more capable proteins/peptides at the single-cell level. Currently, this technology is widely used in the control of environmental pollution. This review is focused on recent strategies of using cell-surface display technology in environmental bioremediation, summarizing its applications, recent progress and future prospects.

Key words: Bioremediation Biosorption Cell surface display Whole cell biocatalyst Microorganism

收稿日期: 2022-05-23 出版日期: 2022-10-10

ZTFLH:

Q813

基金资助: * 国家自然科学基金(31960014);国家自然科学基金(31660019);江西省自然科学基金(20202ACBL205001);江西省教育厅科技项目(GJJ210321)

通讯作者: 陈雪岚 E-mail: xuelanchen162@163.com

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引用本文:

黄明珠,姚坤,宋卓琳,张豪,刘斌,陈雪岚. 细胞表面展示技术在环境修复方面的研究进展^*[J]. 中国生物工程杂志, 2022, 42(9): 105-115.

HUANG Ming-zhu,YAO Kun,SONG Zhuo-lin,ZHANG Hao,LIU Bin,CHEN Xue-lan. Advances in Cell Surface Display Technology in Environmental Remediation. China Biotechnology, 2022, 42(9): 105-115.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2205046 或 https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I9/105

图1 细胞表面展示系统[5]

图2 合成细菌-MNP协同组装吸附重金属的路线[21]

图3 通过温度控制进行Cu2+的生物检测和生物修复的技术路线[24]

图4 Hg2+生物修复和生物控制系统[25]

表1 近年在环境修复中应用的细胞表面展示系统

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