玉米叶夹角形成的遗传基础与分子机制解析<sup>*</sup>

doi:10.13523/j.cb.2111001

中国生物工程杂志

2021, Vol. 41

Issue (12): 74-87 DOI: 10.13523/j.cb.2111001

玉米生物育种基础研究与关键技术专辑

玉米叶夹角形成的遗传基础与分子机制解析^*

秦文萱^1,²,刘鑫^1,²,龙艳^1,^2,³,董振营^1,^2,^**(

),万向元^1,^2,^3,^**(

)

1 北京科技大学生物与农业研究中心化学与生物工程学院顺德研究生院北京 100083
2 北京中智生物农业国际研究院北京 100192
3 北京首佳利华科技有限公司主要作物生物育种北京市工程实验室生物育种北京市国际科技合作基地北京 100192

Progress on Genetic Analysis and Molecular Dissection on Maize Leaf Angle Traits

QIN Wen-xuan^1,²,LIU Xin^1,²,LONG Yan^1,^2,³,DONG Zhen-ying^1,^2,^**(

),WAN Xiang-yuan^1,^2,^3,^**(

)

1 Research Center of Biology and Agriculture, Shunde Graduate School, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China
2 Zhongzhi International Institute of Agricultural Biosciences, Beijing 100192, China
3 Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding, Beijing International Science and Technology Cooperation Base of Bio-Tech Breeding, Beijing Solidwill Sci-Tech Co., Ltd., Beijing 100192, China

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摘要：

玉米产量取决于植株捕获光能和固定CO₂合成有机化合物的效率。叶夹角是株型重要性状之一,较小叶夹角有利于提高玉米植株光合作用效率和种植密度,因而有利于提高玉米产量。研究表明玉米叶夹角为多基因控制的复杂数量性状,其遗传力较高,主要受基因的加性效应调控。目前,利用数量性状位点(quantitative trait loci, QTL)定位和全基因组关联分析(genome-wide association study, GWAS)等方法已鉴定数百个玉米叶夹角相关QTL;结合突变体分析等方法,已克隆数十个调控叶夹角关键基因,这为了解玉米叶夹角遗传机制提供了重要参考。由于前人研究所采用群体、分析方法及参考基因组版本不同,各研究之间所鉴定QTL差异较大,因此无法客观揭示叶夹角性状的遗传规律。为此,通过总结前人所定位叶夹角相关QTL和单核苷酸多态性(single nucleotide polymorphism,SNP)位点并构建一致性图谱,鉴定出叶夹角性状定位热点区间,并对调控叶夹角的已知基因进行功能分类。这不仅为了解玉米叶夹角的遗传结构、推动叶夹角相关重要基因克隆提供数据支撑,也对进一步开发叶夹角相关分子标记,指导玉米分子育种和提高玉米产量提供有益指导。

关键词： 玉米; 叶夹角; 数量性状位点; 全基因组关联分析; 遗传结构

Abstract:

Maize yield is determined by the efficiency of plant capturing light energy and fixing CO₂ into organic carbon compounds. Leaf angle (LA) is one of the important traits of plant architecture, and smaller leaf angle is beneficial to improving the photosynthetic efficiency, planting density, and ultimately the increase of the yield of maize. Previous studies have shown that LA of maize is a complex quantitative trait controlled by multiple genes with higher heritability and significant contribution of additive genetic effects. At present, hundreds of quantitative trait loci (QTLs) related to LA in maize have been identified by QTL mapping and genome-wide association study (GWAS). Combined with mutant analysis, dozens of key genes regulating LA have also been cloned, which provides an important clue for understanding the genetic mechanism of LA in maize. Previous studies have used different populations, analysis models and even reference genome versions for maize LA genetic analysis, the identified QTLs were quite different in those studies, which hampered the understanding of genetic structure of LA traits. Therefore, in this study, we summarized the mapped QTLs and associated single nucleotide polymorphisms (SNPs) related to LA and constructed a consistent map, and then identified the genetic hot spots of LA. Finally, the cloned functional genes regulating LA were analyzed and classified. Our work not only provides primary data for understanding the genetic structure of maize LA and promoting the cloning of the candidate genes, but also provides useful molecular markers for molecular marker-assisted breeding and maize yield improvement.

Key words: Maize Leaf angle Quantitative trait loci(QTLs) Genome-wide association analysis(GWAS) Genetic structure

收稿日期: 2021-10-31 出版日期: 2022-01-13

ZTFLH:

Q819

基金资助: * 中央高校基本科研业务费专项资金(06500060);国家“万人计划”科技创新领军人才特殊支持经费(201608)

通讯作者: 董振营,万向元 E-mail: zydong@ustb.edu.cn;wanxiangyuan@ustb.edu.cn

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引用本文:

秦文萱,刘鑫,龙艳,董振营,万向元. 玉米叶夹角形成的遗传基础与分子机制解析^*[J]. 中国生物工程杂志, 2021, 41(12): 74-87.

QIN Wen-xuan,LIU Xin,LONG Yan,DONG Zhen-ying,WAN Xiang-yuan. Progress on Genetic Analysis and Molecular Dissection on Maize Leaf Angle Traits. China Biotechnology, 2021, 41(12): 74-87.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2111001 或 https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I12/74

图1 玉米叶夹角性状相关QTLs及SNPs在染色体上的分布

图2 玉米叶夹角性状相关QTL、SNP热点区间及基因在染色体上的分布

途径1	基因名称	基因ID	位置 /bp	编码蛋白及功能注解	参考文献
1	Kn1	Zm00001d033859	Chr1: 276073335~276081242	KNOX转录因子	[60]
	LG1	Zm00001d002005	Chr2: 4230854~4234535	SPL转录因子	[17]
	WAB1	Zm00001d005737	Chr2: 185422359~185423189	TCP家族转录因子	[63]
	LG3	Zm00001d040611	Chr3: 53767845~53777713	KNOX转录因子	[64]
	LG2	Zm00001d042777	Chr3: 179387727~179396447	bZIP转录因子	[65]
	YABBY9	Zm00001d013895	Chr5: 24089489~24093556	C2C2-yabby转录因子	[66]
	YABBY15	Zm00001d017391	Chr5: 194316184~194319929	C2C2-yabby转录因子	[66]
	LBL1	Zm00001d035256	Chr6: 17759389~17765846	基因沉默3同源基因抑制因子	[83]
	RS1	Zm00001d018742	Chr7: 3845026~3851209	KNOX转录因子	[13]
	MWP1	Zm00001d020384	Chr7: 110525083~110531709	KANADI 转录因子	[67]
	LG4	Zm00001d010948	Chr8: 134760586~134766141	KNOX转录因子	[64]
	LGN-R	Zm00001d045945	Chr9: 48527705~48530228	受体样丝氨酸/苏氨酸蛋白激酶	[84]
	RLD1	Zm00001d048527	Chr9: 157815873~157824341	HD-ZIPⅢ蛋白	[20]
	YABBY14	Zm00001d025944	Chr10: 134353026~134355488	C2C2-yabby转录因子	[66]
2	DRL1	Zm00001d028216	Chr1: 26771165~26776231	CRC同源转录调控因子	[56]
	DRL2	Zm00001d048083	Chr9: 149627346~149632516	CRC同源转录调控因子	[56]
3	ZmDWF4	Zm00001d028325	Chr1: 30793737~30799897	甾体22-α-羟化酶	[57]
	ZmPGP1	Zm00001d031871	Chr1: 204748411~204755635	ABC转运蛋白家族成员	[58]
	ZmBRD1	Zm00001d033180	Chr1: 253162491~253166753	油菜素类固醇C-6氧化酶	[59]
	ZmAS1	Zm00001d033981	Chr1: 279982395~279986186	硫酸腺苷酰转移酶	[61]
	ZmIBH1-1	Zm00001d001982	Chr2: 3891754~3892380	bHLH转录因子	[61]
	ZmILI1	Zm00001d002121	Chr2: 6144651~6145983	bHLH转录因子	[62]
	ZmRAVL1	Zm00001d002562	Chr2: 15448580~15449809	B3结构域转录调控因子	[12]
	ZmCLA4	Zm00001d049174	Chr4: 19153020~19159545	EAR结构域转录抑制因子	[51]
	NA2	Zm00001d014887	Chr5: 67026171~67030023	24-亚甲基烯醇异构酶/还原酶	[85]
	DIL1	Zm00001d038087	Chr6: 147379164~147385725	AP2-like转录因子	[86]
	ZmBRI1a	Zm00001d011721	Chr8: 159899428~159902796	油菜素内脂受体蛋白	[87]
	ZmBBC1	Zm00001d011992	Chr8: 166018999~166021525	60S核糖体蛋白L13	[61]
	ZmACS7	Zm00001d026060	Chr10: 137296427~137304511	1-氨基环丙烷-1-羧酸合酶7	[68]
其他	TAC1	Zm00001d006184	Chr2: 200972420~200977073	功能未知功能蛋白质	[49]

表1 已克隆玉米叶夹角性状调控基因信息

表S1 玉米叶夹角QTLs及SNPs汇总和定位热点区间鉴定

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