木聚糖酶异源表达的研究进展 <sup>*</sup>

doi:10.13523/j.cb.20190713

中国生物工程杂志

2019, Vol. 39

Issue (7): 91-99 DOI: 10.13523/j.cb.20190713

综述

木聚糖酶异源表达的研究进展 ^*

李吉萍,包昌杰,陈光(

),张斯童(

)

吉林农业大学生命科学学院长春 130118

Research Advances in Heterologous Expression of Xylanase

Ji-ping LI,Chang-jie BAO,Guang CHEN(

),Si-tong ZHANG(

)

College of Life Sciences, Jilin Agricultural University, Changchun 130118,China

全文: PDF(547 KB) HTML

摘要：

木聚糖(xylan)在自然界中的含量极其丰富,在农作物和农林剩余物中大量存在。随着能源资源问题的日益凸显,对木聚糖的应用和研究越来越受到重视。木聚糖酶(xylanase)是可以将木聚糖降解为低聚木糖和木糖的一类水解酶,近年来,为了实现木聚糖酶的高产、高酶活表达,科研工作者做了大量的研究工作,就木聚糖酶异源表达(heterologous expression)的研究进展进行综述。

关键词： 木聚糖; 木聚糖酶; 异源表达

Abstract:

Xylan is abundant in crops and agricultural and forestry residues. With the increasing emphasis on energy resources, more attention has been paid to the application and research of xylan. Xylanase is a class of hydrolases that can degrade xylan into xylooligosaccharides and xylose. Several improvements were made over the year to achieve high yield and high enzyme activity of xylanase.The research progress of heterologous expression of xylanase were reviewed.

Key words: Xylan Xylanase Heterologous expression

收稿日期: 2018-12-10 出版日期: 2019-08-05

ZTFLH:

Q819

基金资助: * 国家重点研发计划(2017YFD0501000);吉林省秸秆综合利用技术创新平台资助项目(2014【C】-1)

通讯作者: 陈光,张斯童 E-mail: chg61@163.com;18943132269@163.com

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引用本文:

李吉萍,包昌杰,陈光,张斯童. 木聚糖酶异源表达的研究进展 ^*[J]. 中国生物工程杂志, 2019, 39(7): 91-99.

Ji-ping LI,Chang-jie BAO,Guang CHEN,Si-tong ZHANG. Research Advances in Heterologous Expression of Xylanase. China Biotechnology, 2019, 39(7): 91-99.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190713 或 https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I7/91

图1 木聚糖酶3D结构模型[5]

表1 木聚糖酶在细菌表达系统中的表达

表2 木聚糖酶在真菌表达系统中的表达

表3 木聚糖酶在植物表达系统中的表达

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