技术与方法 |
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利用CRISPR/Cas9技术建立敲除JAK2基因K562细胞系 * |
菅璐,黄映辉,梁天亚,王利敏,马洪涛,张婷,李丹阳,王明连() |
北京工业大学 北京 100020 |
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Generation of JAK2 Gene Knockout K562 Cell Line by CRISPR/Cas9 System |
Lu JIAN,Ying-hui HUANG,Tian-ya LIANG,Li-min WANG,Hong-tao MA,Ting ZHANG,Dan-yang LI,Ming-lian WANG() |
Beijing University of Technology, Beijing 100020, China |
引用本文:
菅璐,黄映辉,梁天亚,王利敏,马洪涛,张婷,李丹阳,王明连. 利用CRISPR/Cas9技术建立敲除JAK2基因K562细胞系 *[J]. 中国生物工程杂志, 2019, 39(7): 39-47.
Lu JIAN,Ying-hui HUANG,Tian-ya LIANG,Li-min WANG,Hong-tao MA,Ting ZHANG,Dan-yang LI,Ming-lian WANG. Generation of JAK2 Gene Knockout K562 Cell Line by CRISPR/Cas9 System. China Biotechnology, 2019, 39(7): 39-47.
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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190706
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https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I7/39
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[1] |
Samanta A, Perazzona B, Chakraborty S , et al. Janus kinase 2 regulates Bcr-Abl signaling in chronic myeloid leukemia. Leukemia, 2011,25(3):463-472.
|
[2] |
Chen M, Gallipoli P, Degeer D , et al. Targeting primitive chronic myeloid leukemia cells by effective inhibition of a new AHI-1-BCR-ABL-JAK2 complex. Journal of the National Cancer Institute, 2013,105(6):405-423.
doi: 10.1093/jnci/djt006
|
[3] |
Warsch W, Grundschober E, Berger A , et al. STAT5 triggers BCR-ABL1 mutation by mediating ROS production in chronic myeloid leukaemia. Oncotarget, 2012,3(12):1669-1687.
|
[4] |
James C, Ugo V, Le Couédic J P , et al. A unique clonal JAK2 mutation leading to constitutive signalling causes polycythaemia vera. Nature, 2005,434(7037):1144-1148.
|
[5] |
Hartlerode A J, Scully R . Mechanisms of double-strand break repair in somatic mammalian cells. Biochemical Journal, 2009,423(2):157-168.
doi: 10.1042/BJ20090942
|
[6] |
Chandrasegaran S . Recent advances in the use of ZFN-mediated gene editing for human gene therapy. Cell & Gene Therapy Insights, 2017,3(1):33-41.
|
[7] |
Zhang Z, Wu W S . Application of TALE-Based approach for dissecting functional microRNA-302/367 in cellular reprogramming. Methods Mol Biol, 2018,1733:255-263.
doi: 10.1007/978-1-4939-7601-0
|
[8] |
Jinek M, Chylinski K, Fonfara I , et al. A programmable Dual-RNA-Guided DNA endonuclease in adaptive bacterial immunity. Science, 2012,337(6096):816-821.
doi: 10.1126/science.1225829
|
[9] |
Ran Fa, Hsu P D, Wright J , et al. Genome engineering using the CRISPR-Cas9 system. Nature Protocols, 2013,8(11):2281-2308.
|
[10] |
Shen B . Generation of gene-modified mice via cas9/RNA-mediated gene targeting. Cell Research, 2013,23(5):720-723.
|
[11] |
Niu Y, Shen B, Cui Y , et al. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell, 2014,156(4):836-843.
doi: 10.1016/j.cell.2014.01.027
|
[12] |
Sánchez-Rivera F J, Papagiannakopoulos T, Romero R , et al. Rapid modelling of cooperating genetic events in cancer through somatic genome editing. Nature, 2014,516(7531):428-431.
|
[13] |
Liao H K, Gu Y, Diaz A , et al. Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells. Nature Communications, 2015,6(1):6413.
|
[14] |
Kotterman M A, Schaffer D V . Engineering adeno-associated viruses for clinical gene therapy. Nature Reviews Genetics, 2014,15(7):445-451.
|
[15] |
Xu L, Yang H, Gao Y , et al. CRISPR/Cas9-Mediated CCR5 ablation in Human hematopoietic stem/progenitor cells confers HIV-1 resistance in vivo. Molecular Therapy, 2017,25(8):1782.
doi: 10.1016/j.ymthe.2017.04.027
|
[16] |
Neviani P, Harb J G, Oaks J J , et al. PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells. Journal of Clinical Investigation, 2013,123(10):4144-4157.
doi: 10.1172/JCI68951
|
[17] |
Warsch W, Grundschober E, Sexl V . Adding a new facet to STAT5 in CML:multitasking for leukemic cells. Cell Cycle, 2013,12(12):2.
|
[18] |
Weber A, Borghouts C, Brendel C , et al. Stat5 exerts distinct, vital functions in the cytoplasm and nucleus of Bcr-Abl+ K562 and Jak2(V617F)+ HEL leukemia cells. Cancers, 2015,7(1):503.
doi: 10.3390/cancers7010503
|
[19] |
Gallipoli P, Cook A, Rhodes S , et al. JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of chronic phase CML CD34 + cells in vitro and in vivo. Blood, 2014,124(9):1492-501.
doi: 10.1182/blood-2013-12-545640
|
[20] |
Zetsche B, Gootenberg J S, Abudayyeh O O , et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 2015,163(3):759-771.
doi: 10.1016/j.cell.2015.09.038
|
[21] |
Abudayyeh O O, Gootenberg J S, Essletzbichler P , et al. RNA targeting with CRISPR-Cas13. Nature, 2017,550(7675):280-284.
|
[22] |
Mali P, Aach J, Stranges P B , et al. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nature Biotechnology, 2013,31(9):833-838.
|
[23] |
Rees H A, Komor A C, Yeh W H , et al. Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nature Communications, 2017,8(1):15790.
|
[24] |
Rees H A, Liu D R . Base editing: precision chemistry on the genome and transcriptome of living cells. Nature Reviews Genetics, 2018,19(1):770-788.
doi: 10.1038/s41576-018-0059-1
|
[25] |
Shao Y, Guan Y, Wang L , et al. CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos. Nature Protocols, 2014,9(10):2493-2512.
|
[26] |
Song B, Fan Y, He W , et al. Improved hematopoietic differentiation efficiency of gene-corrected beta-thalassemia induced pluripotent stem cells by CRISPR/Cas9 system. Stem Cells and Development, 2015,24(9):1053-1065.
doi: 10.1089/scd.2014.0347
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