中国生物工程杂志

A novel molecular imprinted electrochemical sensor was prepared by electro-polymerization on the surface of a glassy carbon electrode in NaAc-HAc buffer solution, and enrofloxacin was used as template molecular, o-phenylenediamine (OPD) and o-aminophenol (OAP) as composite functional monomer. The sensor could specifically recognize the template molecules and its analogues. In the experiment, 1 mol/L KCl and 5 mmol/L K₃[Fe(CN)₆] was used as characterization solution, the electrochemical sensor response characteristics were researched, and the preparation and detection conditions of the sensor were optimized by square wave voltammetry (SWV) and cyclic voltammetry (CV). The results showed that under the optimal conditions, the linear relationship between SWV peak current difference and the concentrations of enrofloxacin was found in the range of 2×10 ^-6 mol/L4×10 ^-5 mol/L with the linear correlation coefficient ( R ² ) of 0.9990, the detection limit was 7.0×10 ^-7mol/L, and the sensor had good stability, repeatability and good selectivity to enrofloxacin and its analogues. Enrofloxacin in real samples, such as milk, egg, chicken and pork, was detected by the developed sensor, the average recoveries were between 83.2% and 92.7%, and the relative standard deviations ( RSDs ) were 1.0%~4.8% ( n=5 ). This sensor is simple, sensitive, low cost, and has good selectivity and stability, which provide it a good application prospect.