5例前列腺癌T细胞受体β链CDR3的多样性分析 <sup>*</sup>

doi:10.13523/j.cb.20190302

中国生物工程杂志

2019, Vol. 39

Issue (3): 7-12 DOI: 10.13523/j.cb.20190302

研究报告

5例前列腺癌T细胞受体β链CDR3的多样性分析 ^*

潘卫兵^1,^**(

),朱鹏²,曾启昂²,王凯²,刘松³

Diversity Analysis of 5 CDR3s of T Cell Receptor β Chain in Prostate Cancer

Wei-bing PAN^1,^**(

),Peng ZHU²,Qi-ang ZENG²,Kai WANG²,Song LIU³

Research Center for Clinical Medicine of Shenzhen Hospital, Shenzhen 518118,China

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摘要：

T淋巴细胞受体(TCRs)在抗原识别免疫应答中作用重要,其多样性与宿主免疫反应及肿瘤预后判断密切相关。目的: 采用高通量测序技术研究前列腺癌组织和癌旁组织中T细胞受体(TCR)克隆多样性及其克隆序列。方法: 收集5例PC患者的癌组织和癌旁组织,提取DNA后经多重PCR技术对TCR β链CDR3区进行扩增。用Illumina MiSeq进行测序,经过数据处理和比对分析,比较前列腺癌组织TCR CDR3组库的组成特征。结果: 前列腺癌组织具有更高程度的克隆百分比和较高的高度扩增克隆比HEC(HEC:频率>样本总读数的0.5%的TCR克隆),并获得了24对在癌组织样本中差异表达的V-J基因组合、16对在癌旁组织样本中差异表达的V-J基因组合。结论: 前列腺癌组织与癌旁组织均存在差异性的V-J基因组合以及高克隆表达的HEC,其中癌组织样品具有更高的HEC。PC发生发展对免疫学研究提供了新的数据,对PC免疫监视、T细胞受体变异标志等研究提供了参考,对以后继续研究打下了基础。

关键词： 前列腺癌; Tcell受体; 免疫组库; 高通量测序

Abstract:

T lymphocyte receptors (TCRs) play an important role in antigen recognition immune response, and their diversity is closely related to host immune response and tumor prognosis. Objective: To investigate the clonal diversity and cloning sequence of T cell receptor (TCR) in prostate cancer tissues and paracancerous tissues by high-throughput sequencing. Methods: The cancer tissues and paracancerous tissues of 5 patients with PC were collected.After DNA extraction, the CDR3 region of TCR β chain was amplified by multiplex PCR. Sequencing was performed with Illumina MiSeq, and the composition characteristics of the TCR CDR3 library of prostate cancer tissues were compared by data processing and comparison analysis. Results: Prostate cancer tissues had a higher degree of cloning and higher high-amplification clones than HECs (HEC: frequency>0.5% TCR clones of total sample reads) and obtained 24 pairs of differential expression in cancer tissue samples The VJ gene combination, 16 pairs of VJ gene combinations differentially expressed in paracancerous tissue samples. Conclusion: There are different V-J gene combinations and high cloned HEC in prostate cancer tissues and adjacent tissues, and cancer tissue samples have higher HEC. This study provides new data for immunological research on the development of PC, and provides reference for research on PC immune surveillance and T cell receptor mutation markers, laying a foundation for further research.

Key words: Prostate cancer Tcell receptor Immune pool High-throughput sequencing

收稿日期: 2018-08-09 出版日期: 2019-04-12

ZTFLH:

R737

基金资助: * 深圳市坪山新区医疗卫生发展孵化资助(201608);深圳市科技创新委员会知识创新计划基础研究资助项目(JCYJ20140416095712714)

通讯作者: 潘卫兵 E-mail: 1329700025 @qq.com

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引用本文:

潘卫兵,朱鹏,曾启昂,王凯,刘松. 5例前列腺癌T细胞受体β链CDR3的多样性分析 ^*[J]. 中国生物工程杂志, 2019, 39(3): 7-12.

Wei-bing PAN,Peng ZHU,Qi-ang ZENG,Kai WANG,Song LIU. Diversity Analysis of 5 CDR3s of T Cell Receptor β Chain in Prostate Cancer. China Biotechnology, 2019, 39(3): 7-12.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190302 或 https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I3/7

表1 癌症和癌旁组织中的HEC

图1 癌组织与癌旁组织CDR3碱基序列在各个克隆频率分布段的克隆分布情况

图2 癌症与癌旁组织的V基因比对情况

图3 癌组织与癌旁组织的J 基因比对情况

表2 VJ基因配对在癌症样本中的应用

表3 VJ基因配对在癌旁样本中的应用

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