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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (7): 94-101    DOI: 10.13523/j.cb.20180713
综述     
CRISPR-dCas9调控基因转录的研究进展 *
田开仁1,2,3,薛二淑1,2,3,宋倩倩1,2,3,乔建军1,2,3,李艳妮1,2,**()
1 天津大学化工学院 天津 300072
2 系统生物工程教育部重点实验室 天津 300072
3 天津化学化工协同创新中心合成生物学平台 天津 300072
The Research Progress of CRISPR-dCas9 in Transcriptional Regulation
Kai-ren TIAN1,2,3,Er-shu XUE1,2,3,Qian-qian SONG1,2,3,Jian-jun QIAO1,2,3,Yan-ni LI1,2,**()
1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2 Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China
3 Syn Bio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China
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摘要：

CRISPR/Cas9系统已不仅仅是一种革命性的基因编辑工具,还能在各种原核和真核生物中调控基因转录。近年来,由CRISPR/Cas9衍生而来的CRISPR-dCas9系统已被用于基因成像、高通量筛选、基因调控、必需基因功能研究及表观遗传调控等多个方向。总结了近年来CRISPR-dCas9系统在激活或抑制基因转录、降低脱靶效率、sgRNA与转录强度及特异性之间的联系等方面的研究进展,以期对CRISPR-dCas9系统定向调控基因转录的研究提供参考和帮助,并就其未来可能的改进进行了展望。
关键词： CRISPR-dCas9;  基因调控;  转录激活;  转录抑制    
Abstract:

The CRISPR/Cas9 system is not only a revolutionary tool for gene editing but also regulates gene transcription in various prokaryotic and eukaryotic organisms. In recent years,the system of CRISPR-dCas9 derived from CRISPR/Cas9 has been used in many fields such as gene imaging, high-throughput screening, gene regulation, investigating essential gene function and epigenetic regulation.The recent advances of CRISPR-dCas9 for activating or inhibiting gene transcription, reducing off-targeting efficiency and combing the intrinsic relationship between sgRNA and transcriptional regulation, application in the life science and further upgrading were described.
Key words: CRISPR-dCas9    Transcriptional regulation    Transcriptional activation    Transcriptional inhibition
收稿日期: 2018-03-15 出版日期: 2018-08-13
ZTFLH:  Q789  
基金资助: 国家自然科学基金(31570089)
通讯作者: 李艳妮     E-mail: liyanni@tju.edu.cn
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引用本文:

田开仁,薛二淑,宋倩倩,乔建军,李艳妮. CRISPR-dCas9调控基因转录的研究进展 *[J]. 中国生物工程杂志, 2018, 38(7): 94-101.

Kai-ren TIAN,Er-shu XUE,Qian-qian SONG,Jian-jun QIAO,Yan-ni LI. The Research Progress of CRISPR-dCas9 in Transcriptional Regulation. China Biotechnology, 2018, 38(7): 94-101.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180713    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I7/94

图1  CRISPRa系统的作用机制及不同类型的CRISPRa系统
图2  CRISPRi系统作用机制及不同类型的CRISPRi抑制系统
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