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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2018, Vol. 38 Issue (5): 10-16    DOI: 10.13523/j.cb.20180502
研究报告     
骨形成蛋白9对人膀胱癌BIU-87细胞增殖和迁移的影响
苟理尧,刘梦瑶,夏菁,万群,孙恃雷,唐敏,张彦()
重庆医科大学检验医学院 临床检验诊断学教育部重点实验室 重庆 400016
The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells
Li-yao GOU,Meng-yao LIU,Jing XIA,Qun WAN,Chi-lei SUN,Min TANG,Yan ZHANG()
Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education,Chongqing Medical University, Chongqing 400016, China
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摘要：

目的:人骨形成蛋白9(bone morphogenetic protein 9,BMP9)对人膀胱癌BIU-87细胞增殖和迁移的影响。方法:使用过表达BMP9基因的腺病毒(AdBMP9)感染BIU-87细胞,采用定量PCR检测BMP9 mRNA的表达,Western blot检测BMP9蛋白及BMP9下游相关信号通路蛋白的表达;MTT及集落形成实验检测BIU-87细胞增殖能力;划痕愈合实验及Transwell TM小室迁移实验检测BIU-87细胞迁移能力。结果:感染AdBMP9后,BIU-87细胞中BMP9的mRNA水平和蛋白质水平均显著增加;过表达BMP9后,BIU-87细胞的体外增殖和迁移能力明显增加;Western blot结果显示BMP9可明显激活AKT信号通路。结论:高表达BMP9可能通过激活AKT信号通路促进人膀胱癌BIU-87细胞的增殖和迁移。
关键词： 膀胱癌;  骨形成蛋白9(BMP9);  BIU-87细胞;  细胞增殖;  细胞迁移    
Abstract:

Objective:To investigate the effects of bone morphogenetic protein 9 (BMP9) on the proliferation and migration of human bladder cancer BIU-87 cells.Methods:BIU-87 cells were infected with BMP9 recombinant adenovirus(AdBMP9); The mRNA levels of BMP9 were detected by Q-PCR; The BMP9 protein and its downstream associated signaling pathway protein levels were detected by Western blot; The MTT and colony formation assay were used to test the proliferation of BIU-87 cells; The wound healing and Transwell TM chamber assay were taken to evaluate the migration of BIU-87 cells. Results:The expression of BMP9 in BIU-87 cells was highly increased after AdBMP9 infection; Forced expression of BMP9 promotes both the proliferation and migration of BIU-87 cells; Western blot results showed that BMP9 overexpression also increased AKT phosphorylation.Conclusion:High levels of BMP9 may promote the proliferation and migration of bladder cancer BIU-87 cells by activating the AKT signaling pathway.
Key words: Bladder cancer    BMP9    BIU-87 cells    Cell proliferation    Cell migration
收稿日期: 2017-08-31 出版日期: 2018-06-05
ZTFLH:  Q78  
通讯作者: 张彦     E-mail: zy2753@hotmail.com
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苟理尧
刘梦瑶
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万群
孙恃雷
唐敏
张彦

引用本文:

苟理尧,刘梦瑶,夏菁,万群,孙恃雷,唐敏,张彦. 骨形成蛋白9对人膀胱癌BIU-87细胞增殖和迁移的影响[J]. 中国生物工程杂志, 2018, 38(5): 10-16.

Li-yao GOU,Meng-yao LIU,Jing XIA,Qun WAN,Chi-lei SUN,Min TANG,Yan ZHANG. The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells. China Biotechnology, 2018, 38(5): 10-16.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180502    或    https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I5/10

图1  BMP9在正常膀胱黏膜与膀胱癌中的差异表达
图2  重组BIU-87细胞的构建
图3  BMP9促进膀胱癌BIU-87细胞的增殖
图4  BMP9促进膀胱癌BIU-87细胞的迁移
图5  BMP9激活BIU-87细胞的AKT信号通路
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