γPNA一种新型高效的肽核酸

doi:10.13523/j.cb.20180211

中国生物工程杂志

2018, Vol. 38

Issue (2): 75-81 DOI: 10.13523/j.cb.20180211

综述

γPNA一种新型高效的肽核酸

邱浩,汪铭书,程安春(

)

四川农业大学动物医学院禽病防治研究中心四川农业大学预防兽医研究所动物疫病与人类健康四川省重点实验室成都 611130

γPNA——A New Type of High Efficient Peptide Nucleic Acid

Hao QIU,Ming-shu WANG,An-chun CHENG(

)

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University Institute of Preventive Veterinary Medicine Sichuan Agricultural University Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China

全文: PDF(641 KB) HTML

摘要：

肽核酸(peptide nucleic acid,PNA)是一种人工合成的具有类多肽骨架的DNA类似物,具有与核酸结合特异性强、组织和细胞内生物稳定性好、半衰期长等优点。通过靶向结合DNA/RNA而抑制其复制、转录和翻译过程,进行基因调控。在PNA骨架结构中γ位点引入带手性的官能团,能形成右手螺旋结构,显著提高其与靶DNA/RNA的杂交特性,这种PNA衍生物称之为γPNA。γPNA的溶解性、热稳定性和特异性等化学与生物学特性明显改善,在基因编辑和作为探针检测等方面具有良好的应用前景。通过对γPNA结构、性质及其研究进展进行总结,以期为γPNA反义应用提供理论依据和参考。

关键词： γPNA;; 基因调控; 探针检测; 反义应用

Abstract:

Peptide nucleic acid (PNA) is a synthetic analogue of DNA with the pseudopeptide backbone, which has the advantages of strong binding specificity with nucleic acid, high biological stability in tissues and cells and long half-life.By targeting DNA/RNA binding, PNA could inhibit the replication, transcription and translation of DNA/RNA.Chiral functional groups were introduced at gamma sites of the skeleton structure of PNA to form right-handed helical structures, which significantly improved the hybridization with targeting DNA/RNA, and this PNA derivative was called gamma PNA.The solubility, thermal stability and specificity of gamma PNA have been improved remarkably, and it has a good prospect in gene editing and probe detection. The structure, properties and research progress of gamma PNA are summarized, which provides theoretical basis and references for antisense PNA application.

Key words: γPNA; Gene regulation Probe detection Antisense application

收稿日期: 2017-07-11 出版日期: 2018-03-21

ZTFLH:

Q75

基金资助: 国家自然科学基金(31572521);国家现代农业(水禽)产业技术体系专项(CARS-42-17);动物疫病与人类健康四川省重点实验室专项资助项目(2016JPT0004)

作者简介: 通讯作者程安春, 电子信箱: chenganchun@vip.163.com

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引用本文:

邱浩,汪铭书,程安春. γPNA一种新型高效的肽核酸[J]. 中国生物工程杂志, 2018, 38(2): 75-81.

Hao QIU,Ming-shu WANG,An-chun CHENG. γPNA——A New Type of High Efficient Peptide Nucleic Acid. China Biotechnology, 2018, 38(2): 75-81.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20180211 或 https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I2/75

图1 γPNA的分子结构与螺旋结构

图2 γPNA与DNA杂交复合体结构

图3 γPNA调控翻译

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