中国生物工程杂志

The aim is proposed to understand the application of self-assembling peptide R₂I₄R₂ 3D cell culture human skin fibroblast in vitro and the role of the wound healing process. Circular dichroism was used to analysis its secondary structure influenced by different time,temperature and ion environment; Congo red staining was used to obtain the macro change of R₂I₄R₂ hydrogel; R₂I₄R₂ was used to 3D cell culture material to get the growth state of human skin fibroblast cell strain; AO / EB staining was used to study cell apoptosis in 3D culture environment;SD rat skin wound healing model was founded to check pathological changes by HE staining and immunohistochemical staining. These results indicated that R₂I₄R₂ can form a stable secondary structure in some conditions;24 hours after self-assembling, R₂I₄R₂ can form a homogeneous and stable membrane-like structure for 3D cell culture; Human skin fibroblasts had a hearty growing situation in hydrogel; The data in animal experiments show that R₂I₄R₂ can reduce inflammation, promote neovascularization, accelerate the skin wound repair process. It demonstrate this short peptide can be used to a new material in 3D cell culture and skin trauma repairing and beyond.