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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2017, Vol. 37 Issue (10): 118-125    DOI: 10.13523/j.cb.20171016
综述     
衣藻叶绿体表达重组蛋白及表达优化策略
林优红, 程霞英, 严依雯, 梁宗锁, 杨宗岐
浙江理工大学生命科学学院 杭州 310018
Expression and Optimization Strategy of Recombinant Proteins in Chlamydomonas Chloroplast
LIN You-hong, CHENG Xia-ying, YAN Yi-wen, LIANG Zong-suo, YANG Zong-qi
College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
 全文: PDF(460 KB)   HTML
摘要： 近年来，转基因技术已日趋成熟，医学、工业上的应用也越来越广泛。以重组蛋白为基础的药物治疗是目前医药生产领域发展最快的一项技术。它们的高特异性和低副作用使得治疗效率十分突出。但是重组蛋白表达的复杂性也给生产带来了一定限制。为了促进重组蛋白的应用，人们对适宜其表达的系统和能促进其表达的策略进行了探索。研究发现，衣藻叶绿体作为重组蛋白的生物反应器，能实现重组蛋白快速、高效、低成本生产。同时，衣藻能在人工培养基和人为控制的条件下生长，降低了受污染的风险，与传统的生产系统比较具有不可比拟的优越性。因此，衣藻叶绿体作为医药重组蛋白生物反应器在未来的生物技术领域将发挥巨大作用。
关键词： 重组蛋白;  生物反应器;  表达优化;  衣藻叶绿体    
Abstract: In recent years, transgenic technology has developed rapidly, and it has found an increasingly wide utilization in medical and industrial fields. Drug therapy which based on recombinant proteins is the fastest growing technology in pharmaceutical production areas. Their high specificity and low side effect makes treatment efficiency very prominent. However, the complexity of recombinant proteins synthesis also bring production to a certain restrictions. In order to promote the application of these recombinant proteins, expression systems that fit it and strategies that can facilitate it were explored. Compared to other traditional production systems, the research found that Chlamydomonas chloroplast as a bioreactor has several incomparable advantages, it can achieve more rapidly, high yields, lower cost on the production of recombinant proteins, and can be grown in artificial medium and artificially controlled conditions, which reduce the risk of contamination. Through the application of some new techniques, synthesis of recombinant proteins has also been improved. Therefore,Chlamydomonas chloroplast will play a great role in the field of biotechnology in the future as a recombinant proteins with pharmaceutical relevance bioreactor. The expression of recombinant proteins and the optimization strategies in Chlamydomonas chloroplast were reviewed.
Key words: Algal chloroplast    Optimization strategy    Bioreactor    Recombinant proteins
收稿日期: 2017-04-17 出版日期: 2017-10-25
ZTFLH:  Q819  
基金资助: 浙江省自然科学基金（LY15C020005）、国家自然科学基金（31570370）资助项目
通讯作者: 杨宗岐,yangzongqi@zstu.edu.cn     E-mail: yangzongqi@zstu.edu.cn
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引用本文:

林优红, 程霞英, 严依雯, 梁宗锁, 杨宗岐. 衣藻叶绿体表达重组蛋白及表达优化策略[J]. 中国生物工程杂志, 2017, 37(10): 118-125.

LIN You-hong, CHENG Xia-ying, YAN Yi-wen, LIANG Zong-suo, YANG Zong-qi. Expression and Optimization Strategy of Recombinant Proteins in Chlamydomonas Chloroplast. China Biotechnology, 2017, 37(10): 118-125.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20171016    或    https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I10/118

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