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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2017, Vol. 37 Issue (3): 115-123    DOI: 10.13523/j.cb.20170316
行业分析     
全自动核酸分子诊断系统的现状与发展
杨宇1, 刘雅2, 谷岚2, 赵婷婷1, 任鲁风2
1. 中国检验检疫科学研究院 北京 100123;
2. 中国科学院北京基因组研究所 北京 100101
The Current Situation and the Development Trend of Automatic Nucleic Acid Molecular Diagnostic System
YANG Yu1, LIU Ya2, GU Lan2, ZHAO Ting-ting1, REN Lu-feng2
1. Chinese Academy of Inspection and Quaranine, Beijing 100123, China;
2. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
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摘要:

分子诊断是预测诊断的主要方法,广泛用于血液筛查和传染性疾病的诊断中,也用于个体遗传病的诊断和产前诊断。如今,随着各种新发突发传染病频发,以及精准医疗发展的需要,快速、精确、简便的全自动化集成式分子诊断系统越来越受到全世界的关注。对当前国际致力于全自动化集成式分子诊断系统的公司及其产品进行综述,并对全自动化集成式分子诊断系统的未来发展进行了展望。

关键词: 全自动分子诊断核酸传染病    
Abstract:

Molecular detect methods were widely used in diagnosis of infectious diseases, genetic diseases and prenatal prediction. Nowadays, with the more and more emerging infectious diseases and the development of precision medicine, automated molecular diagnostic system has attracted more and more attention as its rapid, accurate and convenient advantage. The automated molecular diagnostic systems and the company were summarized, and the development trend in this field was prospected.

Key words: Automatic    Nucleic acid    Molecular diagnostics    Infectious diseases
收稿日期: 2016-08-22 出版日期: 2017-03-25
ZTFLH:  Q527  
基金资助:

质检行业专项(201410020),检科院基本科研项目(2016JK031)资助项目

通讯作者: 任鲁风     E-mail: renlf@big.ac.cn
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引用本文:

杨宇, 刘雅, 谷岚, 赵婷婷, 任鲁风. 全自动核酸分子诊断系统的现状与发展[J]. 中国生物工程杂志, 2017, 37(3): 115-123.

YANG Yu, LIU Ya, GU Lan, ZHAO Ting-ting, REN Lu-feng. The Current Situation and the Development Trend of Automatic Nucleic Acid Molecular Diagnostic System. China Biotechnology, 2017, 37(3): 115-123.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20170316        https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I3/115

[1] 刘宾,覃斌兵,刘洪娜,等.全自动核酸分子诊断系统的某些进展.现代科学仪器, 2010, 2:165. Liu B, Qin B B, Liu H N, et al. Some development of fully automatic nucleic acid molecular diagnostic system. Modern Scientific Instruments, 2010, 2:165.
[2] Belgrader P, Young S, Yuan B, et al.A battery-powered notebook thermal cycler for rapid multiplex real-time PCR analysis. Anal Chem, 2001, 73(2):286-289.
[3] McMillan W A. Real-time point-of-care molecular detection of infectious disease agents. American Clinical Laboratory, 2002, 21(1):29-31.
[4] Belgrader P, Hansford D, Kovacs G T, et al.A minisonicator to rapidly disrupt bacterial spores for DNA analysis. Anal Chem,1999, 71(19):4232-4236.
[5] Pinsky B A, Sahoo M K, Sandlund J,et al. Analytical performance characteristics of the CepheidGeneXpert Ebola Assay for the detection of Ebola virus. PLoS One, 2015,10(11):e0142216.
[6] Taylor M T, Belgrader P, Furman B J, et al. Lysing bacterial spores by sonication through a flexible interface in a microfluidic system. Anal Chem,2001, 73(3):492-496.
[7] N'guessan K K, Riccardo A, Dutoziet C C,et al. Genotyping of mutations detected with GeneXpert. Int J Mycobacteriol, 2016,5(2):142-147.
[8] Jang D, Ratnam S, Gilchrist J,et al. Comparison of workflow, maintenance, and consumables in the GeneXpert Infinity 80 and panther instruments while testing for Chlamydia trachomatis and Neisseria gonorrhoeae. Sex Transm Dis, 2016,43(6):377-381.
[9] Semper A E, Broadhurst M J, Richards J,et al. Performance of the GeneXpert Ebola assay for diagnosis of Ebola virus disease in Sierra Leone:a field evaluation study. PLoS Med, 2016,13(3):e1001980.
[10] Ceffa S, Luhanga R, Andreotti M, et al. Comparison of the Cepheid GeneXpert and Abbott M2000 HIV-1 real time molecular assays for monitoring HIV-1 viral load and detecting HIV-1 infection. J Virol Methods, 2016,229:35-39.
[11] Castle P E, Smith K M, Davis T E,et al. Reliability of the Xpert HPV assay to detect high-risk human papillomavirus DNA in a colposcopy referral population. Am J Clin Pathol, 2015,143(1):126-133.
[12] Parcell B J, Ratnayake L, Kaminski G, et al. Value of repeat testing using Cepheid GeneXpertCT/NG for indeterminate PCR results when diagnosing Chlamydia trachomatis and Neisseria gonorrhoeae. Int J STD AIDS, 2015,26(1):65-67.
[13] Buchan B W, Reymann G C, Granato P A,et al. Preliminary evaluation of the research-use-only (RUO)iCubate iC-GPC assay for identification of select gram-positive bacteria and their resistance determinants in blood culture broths. J Clin Microbiol, 2015,53(12):3931-3934.
[14] Date-Chong M, Hudlow W R, Buoncristiani M R,Evaluation of the RapidHITTM 200 and RapidHIT GlobalFiler(®) Express kit for fully automated STRgenotyping. Forensic Sci Int Genet, 2016,23:1-8.
[15] Spizz G, Chen Z, Li P, et al. Determination of genotypes using a fully automated molecular detection system. Arch Pathol Lab Med, 2015,139(6):805-811.
[16] Walker T, Dumadag S, Lee CJ, et al. Clinical impact of laboratory implementation of verigene BC-GN microarray-based assay for detection of gram-begative bacteria in positive blood cultures. J Clin Microbiol. 2016,54(7):1789-1796.
[17] Hill C S. Molecular diagnostic testing for infectious diseases using TMA technology. Expert Review of Molecular Diagnostics, 2001, 1(4):445-455.
[18] Kowalski R P, Karenchak L M, Raju LV, et al.The verification of nucleic acid amplification testing (gen-probe aptima assay) for chlamydia trachomatis from ocular samples. Ophthalmology. 2015,122(2):244-247.
[19] Miller S, Samayoa E, Post L. Development and clinical evaluation of a novel fully automated qualitative PCR assay for the diagnosis of anogenital herpes simplex virus infection. Diagn Microbiol Infect Dis, 2014,80(2):102-106.
[20] Jenkins D M, Kubota R, Dong J, et al. Handheld device for real-time, quantitative, LAMP-based detection of Salmonella enterica using assimilating probes. Biosens Bioelectron, 2011, 30(1):255-260.
[21] Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res, 2000, 28(12):E63.
[22] Focke M, Kosse D, Muller C, et al. Lab-on-a-Foil:microfluidics on thin and flexible films. Lab Chip,2010, 10(11):1365-1386.
[23] Mohammed M I, Desmulliez M P. Lab-on-a-chip based immunosensor principles and technologies for the detection of cardiac biomarkers:a review. Lab Chip,2011, 11(4):569-595.
[24] Xu G, Hsieh T M, Lee D Y, et al. A self-contained all-in-one cartridge for sample preparation and real-time PCR in rapid influenza diagnosis. Lab Chip, 2010, 10(22):3103-3111.
[25] Hurth C, Smith S D, Nordquist A R, et al.An automated instrument for human STR identification:design, characterization, and experimental validation. Electrophoresis, 2010, 31(21):3510-3517.
[26] Du W, Li L, Nichols K P, et al. SlipChip. Lab Chip, 2009, 9(16):2286-2292.
[27] Stedtfeld R D, Tourlousse D M, Seyrig G, et al.Gene-Z:a device for point of care genetic testing using a smartphone. Lab Chip,2012, 12(8):1454-1462.
[28] Scherer J R, Liu P, Mathies R A.Design and operation of a portable scanner for high performance microchip capillary array electrophoresis. Rev Sci Instrum, 2010, 81(11):113105-113107.
[29] Liu P, Li X, Greenspoon S A, et al.Integrated DNA purification, PCR, sample cleanup, and capillary electrophoresis microchip for forensic human identification. Lab Chip,2011, 11(6):1041-1048.
[30] 周晓光, 任鲁风, 李运涛, 等. 下一代测序技术:技术回顾与展望. 中国科学:生命科学, 2010, 40(1):23-37. Zhou X G, Ren L F, Li Y T, et al.The next-generation sequencing technology:a technology review and future perspective. Sci China Life Sci, 2010, 40(1):23-37.

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