研究报告 |
|
|
|
|
稳定表达Aβ特异性单链抗体的哺乳动物细胞株构建和功能研究 |
温杰1, 宋琳琳1, 张莹1, 王荷1, 何金生1, 洪涛1,2 |
1. 北京交通大学理学院生命科学与生物工程研究院 北京 100044;
2. 中国疾病预防控制中心病毒病预防控制所 北京 100052 |
|
Construction and Function of Stable Mammalian Cell Lines Expressing the Aβ-specific Single Chain Fragment Variants |
WEN Jie1, SONG Lin-lin1, ZHANG Ying1, WANG He1, HE Jin-sheng1, HONG Tao1,2 |
1. College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China;
2. Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China |
引用本文:
温杰, 宋琳琳, 张莹, 王荷, 何金生, 洪涛. 稳定表达Aβ特异性单链抗体的哺乳动物细胞株构建和功能研究[J]. 中国生物工程杂志, 2017, 37(2): 1-7.
WEN Jie, SONG Lin-lin, ZHANG Ying, WANG He, HE Jin-sheng, HONG Tao. Construction and Function of Stable Mammalian Cell Lines Expressing the Aβ-specific Single Chain Fragment Variants. China Biotechnology, 2017, 37(2): 1-7.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20170201
或
https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I2/1
|
[1] Tam J H, Pasternak S H. Amyloid and Alzheimer's disease:inside and out. Can J Neurol Sci, 2012, 39(3):286-298.
[2] Bayer A J, Bullock R, Jones R W, et al. Evaluation of the safety and immunogenicity of synthetic Abeta42 (AN1792) in patients with AD. Neurology, 2005, 64(1):94-101.
[3] Fernandez-Funez P, Zhang Y, Sanchez-Garcia J, et al. Anti-Abeta single-chain variable fragment antibodies exert synergistic neuroprotective activities in Drosophila models of Alzheimer's disease. Hum Mol Genet, 2015, 24(21):6093-6105.
[4] Droste P, Frenzel A, Steinwand M, et al. Structural differences of amyloid-beta fibrils revealed by antibodies from phage display. BMC Biotechnol, 2015, 15:57.
[5] Kamynina A V, Holmström K M, Koroev D O, et al. Acetylcholine and antibodies against the acetylcholine receptor protect neurons and astrocytes against beta-amyloid toxicity. Int J Biochem Cell Biol, 2013, 45(4):899-907.
[6] Zhao M, Wang S W, Wang Y J, et al. Pan-amyloid oligomer specific scFv antibody attenuates memory deficits and brain amyloid burden in mice with Alzheimer's disease. Curr Alzheimer Res, 2014, 11(1):69-78.
[7] Zhang Y, Yang H Q, Fang F, et al. Single chain variable fragment against abeta expressed in baculovirus inhibits abeta fibril elongation and promotes its disaggregation. PLoS One, 2015, 10(4):e0124736.
[8] Meli G, Visintin M, Cannistraci I, et al. Direct in vivo intracellular selection of conformation-sensitive antibody domains targeting Alzheimer's amyloid-beta oligomers. J Mol Biol, 2009, 387(3):584-606.
[9] Marin-Argany M, Rivera-Hernández G, Martí J, et al. An anti-Abeta (amyloid beta) single-chain variable fragment prevents amyloid fibril formation and cytotoxicity by withdrawing Abeta oligomers from the amyloid pathway. Biochem J, 2011, 437(1):25-34.
[10] Kim J H, Lee S R, Li L H, et al. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS One, 2011, 6(4):e18556.
[11] Zhang H, Darvell B W. Morphology and structural characteristics of hydroxyapatite whiskers:effect of the initial Ca concentration, Ca/P ratio and pH. Acta Biomater, 2011, 7(7):2960-2968.
[12] Medecigo M, Manoutcharian K, Vasilevko V, et al. Novel amyloid-beta specific scFv and VH antibody fragments from human and mouse phage display antibody libraries. J Neuroimmunol, 2010, 223(1-2):104-114.
[13] Zameer A, Kasturirangan S, Emadi S, et al. Anti-oligomeric Abeta single-chain variable domain antibody blocks Abeta-induced toxicity against human neuroblastoma cells. J Mol Biol, 2008, 384(4):917-928. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|