技术与方法 |
|
|
|
|
α-溶血素的表达及其纳米孔的制备 |
卓丽霞1, 王莹2, 张春萍2, 段静2, 张亚妮1 |
1. 西北大学生命科学学院 西安 710069;
2. 西北大学化学与材料科学学院 西安 710069 |
|
The Expression of α-Hemolysin and the Preparation of Its Nanopore |
ZHUO Li-xia1, WANG Ying2, ZHANG Chun-ping2, DUAN Jing2, ZHANG Ya-ni1 |
1. The College of Life Science, Northwest University, Xi'an 710069, China;
2. College of Chemistry & Mateirals Secience, Northwest University, Xi'an 710069, China |
引用本文:
卓丽霞, 王莹, 张春萍, 段静, 张亚妮. α-溶血素的表达及其纳米孔的制备[J]. 中国生物工程杂志, 2017, 37(1): 53-57.
ZHUO Li-xia, WANG Ying, ZHANG Chun-ping, DUAN Jing, ZHANG Ya-ni. The Expression of α-Hemolysin and the Preparation of Its Nanopore. China Biotechnology, 2017, 37(1): 53-57.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20170108
或
https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I1/53
|
[1] Gray G S, Kehoe M. Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46. Infection and Immunity, 1984, 46(2):615-618.
[2] Tobkes N, Wallace B A, Bayley H. Secondary structure and assembly mechanism of an oligomeric channel protein. Biochemistry, 1985, 24(8):1915-1920.
[3] Song L, Hobaugh M R, Shustak C, et al. Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore. Science, 1996, 274(5294):1859-1866.
[4] Branton D, Deamer D W, Marziali A, et al. The potential and challenges of nanopore sequencing. Nat Biotechnol, 2008, 26(10):1146-1153.
[5] Kang X F, Cheley S, Rice-Ficht A C, et al. A storable encapsulated bilayer chip containing a single protein nanopore. J Am Chem Soc, 2007, 129(15):4701-4705.
[6] Ayub M, Bayley H. Individual RNA base recognition in immobilized oligonucleotides using a protein nanopore. Nano Lett, 2012, 12(11):5637-5643.
[7] Bond P J, Guy A T, Heron A J, et al. Molecular dynamics simulations of DNA within a nanopore:arginine-phosphate tethering and a binding/sliding mechanism for translocation. Biochemistry, 2010, 50(18):3777-3783.
[8] Cracknell J A, Japrung D, Bayley H. Translocating kilobase RNA through the staphylococcal alpha-hemolysin nanopore. Nano Lett, 2013, 13(6):2500-2505.
[9] Yao F, Duan J, Wang Y, et al. Nanopore single-molecule analysis of DNA-doxorubicin interactions. Anal Chem, 2015, 87(1):338-342.
[10] Cheley S, Braha O, Lu X, et al. A functional protein pore with a "retro" transmembrane domain. Protein Sci, 1999, 8(6):1257-1267.
[11] Japrung D, Henricus M, Li Q, et al. Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore. Biophys J, 2010, 98(9):1856-1863.
[12] 王多宁, 赵雁武, 田芙蓉. 考马斯亮蓝微盘比色法测定蛋白质含量. 第四军医大学学报, 2001, 22(6):528-529. Wang D N, Zhao Y W, Tian F R. Protein quantif ication with coomassie bull iant blue microplate-colormetric. J Fourth Mil Med Univ, 2001, 22(6):528-529.
[13] Rotem D, Jayasinghe L, Salichou M, et al. Protein detection by nanopores equipped with aptamers. J Am Chem Soc, 2012, 134(5):2781-2787.
[14] Bayley H. Designed membrane channels and pores. Curr Opin Biotechnol, 1999, 10(1):94-103.
[15] Gu L Q, Dalla Serra M, Vincent J B, et al. Reversal of charge selectivity in transmembrane protein pores by using noncovalent molecular adapters. Proc Natl Acad Sci USA, 2000, 97(8):3959-3964.
[16] Boersma A J, Brain K L, Bayley H. Real-time stochastic detection of multiple neurotransmitters with a protein nanopore. ACS Nano, 2012, 6(6):5304-5308.
[17] Gu L Q, Cheley S, Bayley H. Prolonged residence time of a noncovalent molecular adapter, beta-cyclodextrin, within the lumen of mutant alpha-hemolysin pores. J Gen Physiol, 2001, 118(5):481-494.
[18] Hammerstein A F, Jayasinghe L, Bayley H. Subunit dimers of alpha-hemolysin expand the engineering toolbox for protein nanopores. J Biol Chem, 2011, 286(16):14324-14334.
[19] Mantri S, Sapra K T, Cheley S, et al. An engineered dimeric protein pore that spans adjacent lipid bilayers. Nature Communications, 2013, 4(2):216-219. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|