荒漠微藻的碳氧转换与调控

doi:10.13523/j.cb.20161007

中国生物工程杂志

2016, Vol. 36

Issue (10): 45-52 DOI: 10.13523/j.cb.20161007

研究报告

荒漠微藻的碳氧转换与调控

王彩霞¹, 张腾江², 滕杰², 冯旭东², 李春^1,2

1 石河子大学化学化工学院石河子 832003;
2 北京理工大学生命学院北京 100081

The Efficient Carbon-oxygen Transformation and Regulation of Desert Microalgaes

WANG Cai-xia¹, ZHANG Teng-jiang², TENG Jie², FENG Xu-dong², LI Chun^1,2

1. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;
2. School of Life Science, Beijing Institute of Technology, Beijing 100081, China

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摘要：

为了提高微藻的生物燃料生产效率及其在密闭环境中的碳氧转换效率，以两株荒漠微藻BG18-3、BE6-2和一株淡水蓝藻7924为研究对象，对其进行逆境条件培养，发现荒漠微藻BG18-3在各种逆境中表现最佳。在静态培养中，荒漠微藻BG1-3也具有明显的优势，其生物量干重达到0.26 g/L，硝态氮和磷酸盐去除率分别为36%和99%。在荒漠微藻BG18-3的通气培养中，生物干重量最高（3% CO₂通气培养16天）达到2.63 g/L，生物量产率为164.0 mg/L·d，出口CO₂浓度最低降到0.04%，O₂净含量增加0.68%，这表明荒漠微藻BG18-3具有较高的碳氧转化效率，具有生产生物燃料的潜质。最后根据18s rDNA分析结果将荒漠微藻BG18-3鉴定为栅列藻Scenedesmus littoralis。

关键词： 荒漠微藻; 碳氧转换; 抗逆性

Abstract:

In order to produce biofuels by the use of microalgae and improve the efficiency of carbon-oxygen transfer in a confined environment, the growth of desert microalgae BG18-3, BE6-2 and freshwater cyanobacterial strain 7924 were thoroughly explored in stressed conditions, and microalgae BG18-3 was found to have the best performance in some stressed conditions. Compared to other two strains of microalgae in static culture, desert microalgae BG1-3 had an advantage on microalgae biomass accumulation and nitrogen and phosphorus removal efficiency, of which the biomass dry weight reached 0.26 g/L, nitrogen and phosphate removal efficiency were 36% and 99%, respectively. In the aeration culture of desert microalgae BG18-3 ventilation, the highest biological dry weight (3% CO₂ aeration cultured for 16 days) and the biomass yield reached 2.63 g/L and 164.0 mg/L·d respectively, and the concentration of CO₂ at the outlet dropped to 0.04%, while the concentration of O₂ increased by 0.68%, which indicates that the BG18-3 has the potential to be a biological medium in a confined environment. Finally, the BG18-3 was identified as Scenedesmus littoralis according to the results of rDNA 18s analysis.

Key words: Carbon-oxygen transformation Stress resistance Desert microalgae

收稿日期: 2016-03-25 出版日期: 2016-10-25

ZTFLH:

Q819

基金资助:

国家杰出青年科学基金资助项目（21425624）

通讯作者: 李春,电子信箱:lichun@bit.edu.cn E-mail: lichun@bit.edu.cn

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引用本文:

王彩霞, 张腾江, 滕杰, 冯旭东, 李春. 荒漠微藻的碳氧转换与调控[J]. 中国生物工程杂志, 2016, 36(10): 45-52.

WANG Cai-xia, ZHANG Teng-jiang, TENG Jie, FENG Xu-dong, LI Chun. The Efficient Carbon-oxygen Transformation and Regulation of Desert Microalgaes. China Biotechnology, 2016, 36(10): 45-52.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20161007 或 https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I10/45

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