| 技术与方法 |
|
|
|
|
| 一种适用于片段替换/插入突变扫描的克隆方法 |
| 甘春杨, 刘亚, 罗英英, 张文露, 黄爱龙, 蔡雪飞, 胡接力 |
| 重庆医科大学感染性疾病分子生物学教育部重点实验室 重庆 400016 |
|
| A Cloning Strategy Suitable for DNA Modification by Fragment Scanning |
| GAN Chun-yang, LIU Ya, LUO Ying-ying, ZHANG Wen-lu, HUANG Ai-long, CAI Xue-fei, HU Jie-li |
| Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
引用本文:
甘春杨, 刘亚, 罗英英, 张文露, 黄爱龙, 蔡雪飞, 胡接力. 一种适用于片段替换/插入突变扫描的克隆方法[J]. 中国生物工程杂志, 2016, 36(8): 55-63.
GAN Chun-yang, LIU Ya, LUO Ying-ying, ZHANG Wen-lu, HUANG Ai-long, CAI Xue-fei, HU Jie-li. A Cloning Strategy Suitable for DNA Modification by Fragment Scanning. China Biotechnology, 2016, 36(8): 55-63.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160808
或
https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I8/55
|
| [1] Braman J, Papworth C, Greener A. Site-directed mutagenesis using double-stranded plasmid DNA templates. Methods Mol Biol, 1996, 57:31-44.
[2] Jones D H. PCR mutagenesis and recombination in vivo. PCR Methods & Applications, 1994,3(6):S141-148.
[3] Jones D H, Winistorfer S C. Recombinant circle PCR and recombination PCR for site-specific mutagenesis without PCR product purification. Biotechniques, 1992,12(4):528-530, 532, 534-535.
[4] Qi D, Scholthof K B. A one-step PCR-based method for rapid and efficient site-directed fragment deletion, insertion, and substitution mutagenesis. J Virol Methods, 2008, 149(1):85-90.
[5] Geiser M, Cèbe R, Drewello D, et al. Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase. Biotechniques, 2001, 31(1):88-90, 92.
[6] Stech J, Stech O, Herwig A, et al. Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification. Nucleic Acids Res, 2008,36(21):e139.
[7] Hu J L, Cui J, Guo J J, et al. Phenotypic assay of a hepatitis B virus strain carrying an rtS246T variant using a new strategy. J Med Virol, 2012. 84(1):34-43.
[8] Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision cloning method with high throughput capability. PLoS One, 2008, 3(11):e3647.
[9] Engler C, Gruetzner R, Kandzia R, et al. Golden gate shuffling:a one-pot DNA shuffling method based on type Ⅱs restriction enzymes. PLoS One, 2009,4(5):e5553.
[10] Weber E, Engler C, Gruetzner R, et al. A modular cloning system for standardized assembly of multigene constructs. PLoS One, 2011,6(2):e16765.
[11] Cermak T, Doyle E L, Christian M, et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res, 2011,39(12):e82.
[12] Sanjana N E, Cong L, Zhou Y, et al. A transcription activator-like effector toolbox for genome engineering. Nat Protoc, 2012,7(1):171-192.
[13] Verhaegent M, Christopoulos T K. Recombinant Gaussia luciferase. overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal Chem, 2002,74(17):4378-4385.
[14] Nassal M. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. J Virol, 1992,66(7):4107-4116.
[15] Liu N, Ji L, Maguire M L, et al. cis-Acting sequences that contribute to the synthesis of relaxed-circular DNA of human hepatitis B virus. J Virol, 2004,78(2):642-649.
[16] Lewellyn E B, Loeb D D. Base pairing between cis-acting sequences contributes to template switching during plus-strand DNA synthesis in human hepatitis B virus. J Virol, 2007,81(12):6207-6215. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
