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芋螺毒素MrIA的串联表达、纯化及生物活性鉴定 |
朱晓鹏1,2,3, 张亚宁1, 于津鹏1, 李晓丹1, 罗素兰1,2, 长孙东亭1,2 |
1. 海南大学热带生物资源教育部重点实验室 海口市海洋药物重点实验室 海口 570228;
2. 海南大学海洋学院 海口 570228;
3. 海南大学园艺园林学院 海口 570228 |
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Tandem Expression, Purification and Bioactivity Characterization of Conotoxin MrIA |
ZHU Xiao-peng1,2,3, ZHANG Ya-ning1, YU Jin-peng1, LI Xiao-dan1, LUO Su-lan1,2, ZHANGSUN Dong-ting1,2 |
1. Key Laboratory of Tropical Biological Resources, Ministry of Education, Key Laboratory for Marine Drugs of Haikou, Hainan University, Haikou 570228, China;
2. College of Marine Science, Hainan University, Haikou 570228, China;
3. College of Horticulture and Landscapes, Hainan University, Haikou 570228, China |
引用本文:
朱晓鹏, 张亚宁, 于津鹏, 李晓丹, 罗素兰, 长孙东亭. 芋螺毒素MrIA的串联表达、纯化及生物活性鉴定[J]. 中国生物工程杂志, 2016, 36(5): 81-88.
ZHU Xiao-peng, ZHANG Ya-ning, YU Jin-peng, LI Xiao-dan, LUO Su-lan, ZHANGSUN Dong-ting. Tandem Expression, Purification and Bioactivity Characterization of Conotoxin MrIA. China Biotechnology, 2016, 36(5): 81-88.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160512
或
https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I5/81
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[1] Norton R S, Olivera B M. Conotoxins down under. Toxicon,2006,48(7):780-798.
[2] Olivera B M. Conus peptides:biodiversity-based discovery and exogenomics. J Biol Chem,2006,281(42):31173-31177.
[3] Olivera B M. E.E.Just Lecture,1996.Conus venom peptides, receptor and ion channel targets, and drug design: 50 million years of neuropharmacology. Mol Biol Cell,1997,8(11):2101-2109.
[4] Davis J, Jones A, Lewis R J. Remarkable inter-and intra-species complexity of conotoxins revealed by LC/MS. Peptides,2009,30(7):1222-1227.
[5] Olivera B M, Rivier J, Clark C, et al.Diversity of Conus neuropeptides. Science,1990,249(4966):257-263.
[6] Robinson S D, Norton R S. Conotoxin gene superfamilies. Mar Drugs,2014,12(12):6058-6101.
[7] McIntosh J M, Corpuz G O, Layer R T, et al. Isolation and characterization of a novel conus peptide with apparent antinociceptive activity. J Biol Chem,2000,275(42):32391-32397.
[8] Balaji R A, Ohtake A, Sato K,et al. lambda-conotoxins, a new family of conotoxins with unique disulfide pattern and protein folding. Isolation and characterization from the venom of Conus marmoreus. J Biol Chem,2000,275(50):39516-39522.
[9] Goddard A W, Ball S G, Martinez J, et al. Current perspectives of the roles of the central norepinephrine system in anxiety and depression. Depress Anxiety,2010,27(4):339-350.
[10] Nielsen C K, Lewis R J, Alewood D, et al. Anti-allodynic efficacy of the chi-conopeptide, Xen2174, in rats with neuropathic pain. Pain,2005,118(1-2):112-124.
[11] Lewis R J, Dutertre S, Vetter I, et al. Conus venom peptide pharmacology. Pharmacol Rev,2012,64(2):259-298.
[12] Casarrubea M, Sorbera F, Santangelo A, et al. The effects of diazepam on the behavioral structure of the rat's response to pain in the hot-plate test: anxiolysis vs. pain modulation. Neuropharmacology,2012,63(2):310-321.
[13] Lewis R J. Discovery and development of the χ-conopeptide class of analgesic peptides. Toxicon,2012,59(4):524-528.
[14] Brust A, Palant E, Croker D E, et al. chi-Conopeptide pharmacophore development: toward a novel class of norepinephrine transporter inhibitor (Xen2174) for pain. J Med Chem,2009,52(22):6991-7002.
[15] Kumar G S, Ramasamy P, Sikdar S K, et al. Overexpression, purification, and pharmacological activity of a biosynthetically derived conopeptide. Biochem Biophys Res Commun,2005,335(3):965-972.
[16] Bruce C, Fitches E C, Chougule N, et al. Recombinant conotoxin, TxVIA, produced in yeast has insecticidal activity. Toxicon,2011,58(1):93-100.
[17] Hruby V J. Designing peptide receptor agonists and antagonists. Nat Rev Drug Discov,2002,1(11):847-858.
[18] Sharpe I A, Palant E, Schroeder C I,et al. Inhibition of the norepinephrine transporter by the venom peptide chi-MrIA. Site of action, Na+ dependence, and structure-activity relationship. J Biol Chem,2003,278(41):40317-40323.
[19] Kuliopulos A, Walsh C T. Production, purification, and cleavage of tandem repeats of recombinant peptides. J Am Chem Soc,1994,116(11):4599-4607.
[20] Hwang P M, Pan J S, Sykes B D.Targeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli. FEBS Lett,2014,588(2):247-252. |
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