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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2015, Vol. 35 Issue (12): 89-95    DOI: 10.13523/j.cb.20151214
综述     
LAMP-LFD技术及其在生物快检方面应用
黄海龙1, 朱鹏1, 杨浩3
1. 浙江省宁波大学应用海洋生物技术教育部重点实验室 宁波 315211;
2. 宁波博奥生物工程有限公司 宁波 315211;
3. 中国人民解放军后勤工程学院 重庆 401331
LAMP-LFD Technology and Its Application in Rapid Detection of Biological
HUANG Hai-long1, ZHU Peng1, YANG Hao3
1. Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China;
2. Ningbo Boao Biological Engineering Co., Ltd. Ningbo 315211, China;
3. Dept of Oil Application & Management Engineering, LEU, Chongqing 401311, China
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摘要：

LAMP-LFD技术是环介导横温扩增技术(Loop-mediated isothermal amplification)与横向流动试纸条(Lateral flow dipstick)相结合的一种新颖的检测技术,该技术将核酸横温扩增和可视化试纸条检测有机地结合。环介导等温扩增技术(LAMP),只需要在恒温(60℃~65℃)条件下保温几十分钟,即可将所需目的基因扩增到109水平。横向流动试纸条(LFD),融合了免疫层析技术和分子生物学手段,能在纸条上形成有颜色的检测线从而可以特异性地检测出该目标产物。环介导横温扩增技术(LAMP)与横向流动试纸条(LFD)相互结合的技术,使LAMP扩增产物现场检测可视化,检测结果明显直观,通过肉眼即可辨认,并具有高特异性、高灵敏度、简便、安全及成本低的特点,一度引起科研工作者的广泛关注。综述了环介导横温扩增方法与横向流动试纸条相结合(LAMP-LFD)技术的原理、优势、及其在生物快速检测方面的应用等,并指出了LAMP-LFD技术目前存在的不足以及展望。
关键词： LAMP-LFD技术;  综述;  快速检测    
Abstract:

Loop-mediated isothermal amplification (LAMP) combined with the chromatographic lateral flow dipstick (LFD) assay to detect the target amplicons is a novel detection method. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technology. The biggest advantages of this method is able to amplify the specific target sequences to 109 level under the condition of 60℃~65℃ isothermal temperature within 30~60 minutes.Lateral flow dipstick (LFD), is a blend of immune chromatography technology and molecular biology method,which can make the target product easily to be detected specificity by the color lines in the note. The developed LAMP-LFD assay has the characteristics of high specificity, sensitivity, simplicity, convenience and low cost.What is more,the results can be recognized visually and intuitively by the naked eye.The principle,in rapid detection of biological application and technological characteristics of LAMP-LFD were reviewed, and points out the advantages and existing problems of LAMP-LFD technology.Finally, the LAMP-LFD technology is prospected.
Key words: Rapid detection    LAMP-LFD technology    Review
收稿日期: 2015-07-08 出版日期: 2015-12-22
ZTFLH:  Q819  
基金资助:

浙江省重大科技专项重点农业项目(2012C12009-4)、出入境主要农产品快速检测关键技术研究与示范(2015C110018)资助项目
通讯作者: 朱鹏     E-mail: zhupeng@nbu.edu.cn
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引用本文:

黄海龙, 朱鹏, 杨浩. LAMP-LFD技术及其在生物快检方面应用[J]. 中国生物工程杂志, 2015, 35(12): 89-95.

HUANG Hai-long, ZHU Peng, YANG Hao. LAMP-LFD Technology and Its Application in Rapid Detection of Biological. China Biotechnology, 2015, 35(12): 89-95.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20151214    或    https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I12/89

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