Please wait a minute...

中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2015, Vol. 35 Issue (11): 46-51    DOI: 10.13523/j.cb.20151107
研究报告     
葡萄糖对单针藻异养、兼养生长及油脂合成的影响
车绕琼, 黄力, 王琳, 赵鹏, 李涛, 余旭亚
昆明理工大学生命科学与技术学院 昆明 650500
Effects of Glucose on Cell Growth and Lipid Synthesis of Monoraphidium sp. FXY-10 Under Mixotrophic and Heterotrophic Cultivations
CHE Rao-qiong, HUANG Li, WANG Lin, ZHAO Peng, LI Tao, YU Xu-ya
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
 全文: PDF(653 KB)   HTML
摘要:

利用Andrew方程考查了异养和兼养培养模式下葡萄糖对单针藻Monoraphidium sp. FXY-10生长的影响,拟合出葡萄糖消耗动力学模型;同时研究了不同培养模式下葡萄糖对单针藻油脂合成的影响,拟合出最佳葡萄糖浓度时油脂合成动力学模型。结果表明,葡萄糖浓度为10.0g/L时,异养、兼养培养条件下藻细胞生物量和油脂含量达到最大值;异养、兼养培养条件下葡萄糖的半饱和常数KS分别为17.82g/L和1.89g/L,说明兼养培养时单针藻对葡萄糖的吸收能力相对较高。

关键词: 兼养单针藻油脂含量异养葡萄糖    
Abstract:

The effects of initial glucose concentrations on cell growth of Monoraphidium sp.FXY-10 under mixotrophic and heterotrophic cultivations were investigated by Andrew equation.A kinetic model was developed to describe the dynamics of glucose consumption for FXY-10 growth. Meanwhile,the influence of different glucose concentrations on the production of lipid by FXY-10 was studied. And the kinetic models of FXY-10 lipid synthesis in the different trophic types with the most appropriate glucose concentration were also constructed. The results showed thatwhen the concentration of glucose was 10g/L, the maximum total lipid content and biomass concentration were achieved under mixotrophic and heterotrophic conditions. The semi-saturation constants of Monoraphidium sp. FXY-10 to glucose in the mixotrophic(KSJ) and heterotrophic (KSY)conditions were 1.89g/L and 17.82g/L, respectively. It implied that the glucose absorption capacity of FXY-10 in the mixotrophic condition was relatively higher than that in the heterotrophic.

Key words: Monoraphidium sp. FXY-10    Heterotrophic    Mixotrophic    Glucose    Lipid content
收稿日期: 2015-06-29 出版日期: 2015-11-25
ZTFLH:  Q493.4  
基金资助:

国家自然科学基金资助项目(21266013)

通讯作者: 余旭亚     E-mail: xuya_yu@163.com
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  

引用本文:

车绕琼, 黄力, 王琳, 赵鹏, 李涛, 余旭亚. 葡萄糖对单针藻异养、兼养生长及油脂合成的影响[J]. 中国生物工程杂志, 2015, 35(11): 46-51.

CHE Rao-qiong, HUANG Li, WANG Lin, ZHAO Peng, LI Tao, YU Xu-ya. Effects of Glucose on Cell Growth and Lipid Synthesis of Monoraphidium sp. FXY-10 Under Mixotrophic and Heterotrophic Cultivations. China Biotechnology, 2015, 35(11): 46-51.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20151107        https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I11/46

[1] Chen C Y, Yeh K L, Aisyah R, et al. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresource Technology, 2011, 102(1): 71-81.
[2] Wang J, Yang H, Wang F. Mixotrophic cultivation of microalgae for biodiesel production: status and prospects. Applied Biochemistry and Biotechnology, 2014, 172(7): 3307-3329.
[3] Zhao G L, Yu J Y, Jiang F F, et al. The effect of different trophic modes on lipid accumulation of Scenedesmus quadricauda. Bioresource Technology, 2012, 114(3): 466-471.
[4] Lin T S, Wu J Y. Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition. Bioresource Technology, 2015,184:100-107.
[5] Cheng Y, Lu Y, Gao C, et al. Alga-based biodiesel production and optimization using sugar cane as the feedstock. Energy & Fuels, 2009, 23(8): 4166-4173.
[6] 王琳, 余旭亚, 徐军伟. 氮, 磷对单针藻兼养生长与油脂合成的影响. 化学工程, 2013, 41(2): 1-5. Wang L, Yu X Y, Xu J W. Effects of nitrogen and phosphorus on growth and lipid synthesis of Monoraphidium sp. under mixotrophic cultivation. Chemical Engineering, 2013, 41(2): 1-5.
[7] Yang J S, Rasa E, Tantayotai P, et al. Mathematical model of Chlorella minutissima UTEX2341 growth and lipid production under photoheterotrophic fermentation conditions. Bioresource Technology, 2011, 102(3): 3077-3082.
[8] Liu J Z, Weng L P, Zhang Q L, et al. A mathematical model for gluconic acid fermentation by Aspergillus niger. Biochemical Engineering Journal, 2003, 14(2): 137-141.
[9] 黄力, 贺赐安, 赵鹏, 等. 碳源, 氮源对异养单针藻Monoraphidium sp. FXY-10 油脂积累和脂肪酸组成的影响. 中国生物工程杂志, 2013, 33(2): 59-64. Huang L, He C A, Zhao P, et al. Effect of carbon, nitrogen sources on lipid accumulation and fatty acid composition of Monoraphidium sp. FXY-10 under Heterotrophic Cultivation. China Biotechnology, 2013, 33(2):59-64.
[10] Rai M P, Nigam S, Sharma R. Response of growth and fatty acid compositions of Chlorella pyrenoidosa under mixotrophic cultivation with acetate and glycerol for bioenergy application. Biomass and Bioenergy, 2013, 58: 251-257.
[11] Miao X, Wu Q. High yield bio-oil production from fast pyrolysis by metabolic controlling of Chlorella protothecoides. Journal of Biotechnology, 2004, 110(1): 85-93.
[12] Garcí M C C, Sevilla J M F, Fernández F G A, et al. Mixotrophic growth of Phaeodactylum tricornutum on glycerol: growth rate and fatty acid profile. Journal of Applied Phycology, 2000, 12(3-5): 239-248.
[13] Woodworth B D, Mead R L, Nichols C N, et al. Photosynthetic light reactions increase total lipid accumulation in carbon-supplemented batch cultures of Chlorella vulgaris. Bioresource Technology, 2015, 179: 159-164.
[14] Yeh K L, Chang J S. Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31. Bioresource Technology, 2012, 105: 120-127.
[15] Yang C, Hua Q, Shimizu K. Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic light-autotrophic/dark-heterotrophic conditions. Biochemical Engineering Journal, 2000, 6(2): 87-102.
[16] 赫冬梅, 王娜, 孙凯峰, 等. 葡萄糖和乙酸钠对湛江等鞭金藻兼养生长的影响. 生态科学, 2012, 31(2): 220-223. He D M, Wang N, Sun K F, et al. Effect of glucose and sodium acetate on mixtrophic growth of Isochrysis zhanjiangensis. Ecological Science, 2012,31(2): 220-223.
[17] Ngangkham M, Ratha S K, Prasanna R, et al. Biochemical modulation of growth, lipid quality and productivity in mixotrophic cultures of Chlorella sorokiniana. Springer Plus, 2012, 1(3): 33.
[18] Yu X, Zhao P, He C, et al. Isolation of a novel strain of Monoraphidium sp. and characterization of its potential application as biodiesel feedstock. Bioresource Technology, 2012, 121: 256-262.
[19] Heredia-Arroyo T, Wei W, Ruan R, et al. Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass and Bioenergy, 2011, 35(5): 2245-2253.
[20] Perez-Garcia O, Escalante F M E, de-Bashan L E, et al. Heterotrophic cultures of microalgae: metabolism and potential products. Water Research, 2011, 45(1): 11-36.
[21] Li T, Zheng Y, Yu L, et al. Mixotrophic cultivation of a Chlorella sorokiniana strain for enhanced biomass and lipid production. Biomass and Bioenergy, 2014, 66: 204-213.
[22] Sasaki Y, Kozaki A, Hatano M. Link between light and fatty acid synthesis: thioredoxin-linked reductive activation of plastidic acetyl-CoA carboxylase. Proceedings of the National Academy of Sciences, 1997, 94(20): 11096-11101.

[1] 谢琳娜,卜静静,郑敏. AUF1在胞质DNA抑制细胞葡萄糖代谢中的作用研究[J]. 中国生物工程杂志, 2019, 39(11): 54-61.
[2] 党诗莹,马义,文涛,肖兴,洪岸. 纳米复合肽SCM的制备及其对II型糖尿病治疗作用的研究 *[J]. 中国生物工程杂志, 2018, 38(5): 17-23.
[3] 来亚鹏, 邓婷婷, 刘刚, 王娟. 同源过表达BglR对嗜热毁丝霉β-葡萄糖苷酶活性的影响[J]. 中国生物工程杂志, 2017, 37(7): 64-71.
[4] 王得华, 马义, 韩磊, 肖兴, 李艳伟, 党诗莹, 范志勇, 文涛, 洪岸. 新型基因重组PACAP衍生物MPL-2的制备及其抗2型糖尿病作用研究[J]. 中国生物工程杂志, 2017, 37(5): 59-65.
[5] 翟兵兵, 马倩, 丁明珠, 元英进. 谷胱甘肽对VC一步发酵作用的研究[J]. 中国生物工程杂志, 2016, 36(8): 38-45.
[6] 翟兵兵, 董秀涛, 丁明珠, 元英进. VC三菌种一步发酵方法的构建与研究[J]. 中国生物工程杂志, 2016, 36(12): 72-78.
[7] 郭永华, 陈济琛, 蔡海松, 陈龙军, 林新坚. Geobacillus sp.CHB1环糊精葡萄糖基转移酶淀粉结合位点N623饱和突变对催化效率与产物特异性的影响[J]. 中国生物工程杂志, 2016, 36(11): 30-38.
[8] 王浩, 张敬书, 丁健, 罗洪镇, 陈锐, 史仲平. 限制葡萄糖、葡萄糖/乙酸双底物条件下自由控制丙丁梭菌ABE发酵丙酮浓度和丙酮/丁醇比[J]. 中国生物工程杂志, 2016, 36(10): 60-71.
[9] 刘阳, 杨雅麟, 张宇婷, 冉超, 周志刚. 维氏气单胞菌B565β-N-乙酰氨基葡萄糖苷酶的表达、纯化及酶学性质[J]. 中国生物工程杂志, 2015, 35(2): 38-44.
[10] 夏亚穆, 李晨晨. 环糊精葡萄糖基转移酶的基因改造与高效表达[J]. 中国生物工程杂志, 2015, 35(2): 105-110.
[11] 仪修南, 李天明, 王北辰, 刘金雷, 杜红燕, 冯惠勇. 代谢工程改造Gluconobacter suboxydans生产2-酮基-D-葡萄糖酸[J]. 中国生物工程杂志, 2014, 34(12): 97-106.
[12] 蔡晶晶, 段学辉, 谢亮, 郑希帆, 沈江涛. 三菌混合固态发酵产纤维素酶[J]. 中国生物工程杂志, 2013, 33(7): 57-63.
[13] 汪桂林, 桂小华, 邓伟, 赵志良, 姚杰, 闫云君. “异养-胁迫”分段培养对原始小球藻生物量和油脂含量影响研究[J]. 中国生物工程杂志, 2013, 33(3): 99-104.
[14] 黄力, 贺赐安, 赵鹏, 余旭亚. 碳源、氮源对异养单针藻Monoraphidium sp. FXY-10油脂积累和脂肪酸组成的影响[J]. 中国生物工程杂志, 2013, 33(2): 59-64.
[15] 邓辉, 陈晟, 陈坚, 吴敬. T26P和A30P位点突变对Thermobifida fusca葡萄糖异构酶热稳定性及活性的影响[J]. 中国生物工程杂志, 2013, 33(10): 67-72.