氢化酶重组表达研究进展

doi:10.13523/j.cb.20150516

中国生物工程杂志

2015, Vol. 35

Issue (5): 109-118 DOI: 10.13523/j.cb.20150516

综述

氢化酶重组表达研究进展

丁一^1,3, 吴海英^1,2, 史吉平^1,2, 孙俊松^1,2

1. 中国科学院上海高等研究院生物炼制实验室上海 201210;
2. 上海科技大学生命科学学院上海 201210;
3. 中国科学院大学北京 100049

Current Progress in Recombinant Systems for Expression of Hydrogenases

DING Yi^1,3, WU Hai-ying^1,2, SHI Ji-ping^1,2, SUN Jun-song^1,2

1. Biorefinery Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China;
2. School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China

全文: PDF(474 KB) HTML

摘要：

氢化酶催化最简单的氧化还原反应,但蛋白结构却非常复杂,对其蛋白结构和催化功能的研究牵动着生物制氢、光电产氢催化剂及氢能源电池等相关绿色能源产业的发展。氢化酶通常可逆地催化质子还原产氢的反应,对氧化还原电位非常敏感,催化活性中心易于被氧化失活,活性蛋白的分离提纯十分不易,使得对其催化机制的认识推进缓慢。为了获取更多的氢化酶活性蛋白,许多研究团队先后对氢化酶开展了大量的同源或异源重组表达研究,就这类研究工作进行了扼要的总结和分析。

关键词： 氢化酶; 重组表达; 成熟因子; 翻译修饰

Abstract:

Hydrogenase enzymes, which catalyze the formation and dissociation of hydrogen are heteromeric metalloenzymes. Mature hydrogenases are usually highly sensitive to oxygen,and the pro-enzymes are not active unless they are modified by a complicated post-transltational maturation process which involves synergized work on catalytic center of the enzymes by related chaprons. Catalytic mechanisms of hydrogenases also plays pivotal role in development of valuable oxygen-resistant biocatalysts for bio-hydrogen production and synthetic hydrogenase mimics applied in green battery industry. Recombinant enzymes are therefore indispensable for enzymes' structural studies, since acquisition of native enzymes is extremely difficult, and, in some case impossible. This review aims to summarize and analyze recent progress in studies using native or foreign hosts to achive successful expression of recombinant enzymes that are either iron only or NiFe containing. Furthermore, the enzymatic features were systematically compared between native and recombinant proteins, and the likely solutions for future works in this area were also proposed.

Key words: Hydrogenase Recombinant expression Maturation factor Post-translational modification

收稿日期: 2015-02-03 出版日期: 2015-05-25

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通讯作者: 孙俊松 E-mail: sunjs@sari.ac.cn

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引用本文:

丁一, 吴海英, 史吉平, 孙俊松. 氢化酶重组表达研究进展[J]. 中国生物工程杂志, 2015, 35(5): 109-118.

DING Yi, WU Hai-ying, SHI Ji-ping, SUN Jun-song. Current Progress in Recombinant Systems for Expression of Hydrogenases. China Biotechnology, 2015, 35(5): 109-118.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150516 或 https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I5/109

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