重组大肠杆菌利用废纸水解液发酵产L-乳酸

doi:10.13523/j.cb.20150507

中国生物工程杂志

2015, Vol. 35

Issue (5): 49-54 DOI: 10.13523/j.cb.20150507

研究报告

重组大肠杆菌利用废纸水解液发酵产L-乳酸

熊圆圆, 卢传东, 陶冶, 赵锦芳

工业发酵湖北省协同创新中心发酵工程教育部重点实验室湖北工业大学轻工学部武汉 430068

Fermentative Production of L-lactic Acid from Wastepaper by Recombinant Escherichia coli WL204

XIONG Yuan-yuan, LU Chuan-dong, TAO Ye, ZHAO Jin-fang

Hubei Cooperative Innovation Center for Industrial Fermentation, Key Laboratory of Fermentation Engineering Ministry of Education, Hubei University of Technology, Wuhan 430068, China

全文: PDF(538 KB) HTML

摘要：

前期通过基因工程手段,构建了一株大肠杆菌工程菌E. coli WL204,该菌株可以有效利用木糖为底物发酵产L-乳酸。以废纸为发酵原料,研究该菌株利用木质纤维素发酵产乳酸的特性。原料以稀硫酸预处理后,经纤维素酶酶解,得到的水解液用Ca(OH)₂脱毒后,接种 E. coli WL204,在7L发酵罐中发酵72h,每100g废纸可以产生31g乳酸,糖酸转化率为79%。结果表明,E. coli WL204可以木质纤维素原料为底物发酵生产L-乳酸,具有一定的工业化开发潜力。

关键词： 大肠杆菌; L-乳酸; 废纸; 木质纤维素

Abstract:

Previous studies, a recombinant E.coli WL204 was constructed,which contained a chromosomal integrated ldhL gene and possesses the capability of homofermentation of L-lactic acid using xylose as substrate. Wastepaper was selected as feedstock to evaluate the fermentative production of lactic acid by recombinant E.coli WL204.The wastepaper chips were treated with 1:6-1:14(w/v)H₂SO₄, and then incubated 60 h at 50℃ with cellulase. The resultant hydrolyzates were detoxified with dried Ca(OH)₂, which could remove most of furfural and HMF. Fermentation of the detoxified hydrolyzate of wastepaper with E.coli WL204 produced 31g L-lactic acid from 100g dry wastepaper and the lactic acid yield coefficient was 79%.These results demonstrated that WL204 has the potential for homofermentative production of L-lactic acid using cellulosic biomass derived substrates.

Key words: Escherichia coli L-lactic acid Waste paper Lignocellulose

收稿日期: 2015-03-04 出版日期: 2015-05-25

ZTFLH:

Q815

基金资助:

湖北自然科学基金资助项目(2011CDB076)

通讯作者: 赵锦芳 E-mail: jinfangzhao@126.com

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引用本文:

熊圆圆, 卢传东, 陶冶, 赵锦芳. 重组大肠杆菌利用废纸水解液发酵产L-乳酸[J]. 中国生物工程杂志, 2015, 35(5): 49-54.

XIONG Yuan-yuan, LU Chuan-dong, TAO Ye, ZHAO Jin-fang. Fermentative Production of L-lactic Acid from Wastepaper by Recombinant Escherichia coli WL204. China Biotechnology, 2015, 35(5): 49-54.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150507 或 https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I5/49

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