技术与方法 |
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基于Loxp-Cre系统的FBXL15基因敲除小鼠模型的建立 |
陈静静1,2, 邢桂春2, 张令强1,2 |
1. 安徽医科大学研究生学院 合肥 230032;
2. 军事医学科学院放射与辐射医学研究所 北京 100850 |
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Generation of FBXL15 Conditional Knockout Mice Using the Loxp-Cre Strategy |
CHEN Jing-jing1,2, XING Gui-chun2, ZHANG Ling-qiang1,2 |
1. Graduate School, Anhui Medical University, Hefei 230032, China;
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 100850, China |
[1] Staub M S A O. HECT E3s and human disease. BMC Biochemistry,2007,8(Suppl 1):S6:1471-2091.
[2] Louise Madsen A S, Petersen M S. Ubiquitin domain proteins in disease. BMC Biochemistry, 2007, 8(Suppl 1):S1:1-14.
[3] Schulman B A, Harper J W. Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways. Nature Reviews Molecular Cell Biology, 2009. 10(5):319-331.
[4] Ye Y, Rape M. Building ubiquitin chains: E2 enzymes at work. Nature Reviews Molecular Cell Biology, 2009,10(11):755-764.
[5] Deshaies R J, Ring J. domain E3 ubiquitin ligases. Annual Review of Biochemistry, 2009,78(1):399-434.
[6] Nalepa G, Rolfe M, Harper J W. Drug discovery in the ubiquitin-proteasome system. Nature Reviews Drug Discovery, 2006,5(7):596-613.
[7] Lin H, Wang G, Chen Z, et al. Phosphorylation-dependent regulation of cytosolic localization and oncogenic function of Skp2 by Akt/PKB. Nature Cell Biology, 2009,11(4):420-432.
[8] Jennifer Harrell, Jonason N G. Regulation of SCFSKP2 ubiquitin E3 ligase assembly and p27KIP1 proteolysis by the PTEN pathway and cyclin D1.Cell Cycle,2007,6(8):951-961.
[9] Lin H, Chen Z,Wang G,et al. Skp2 targeting suppresses tumorigenesis by Arf-p53-independent cellular senescence. Nature, 2010,464(7287):374-379.
[10] Chan C H, Li C F,Yang W L,et al. The Skp2-SCF E3 ligase regulates Akt ubiquitination, glycolysis, herceptin sensitivity, and tumorigenesis. Cell, 2012,149(5):1098-1110.
[11] Cui Y, He S, Xing C C,et al. SCFFBXL(1)(5) regulates BMP signalling by directing the degradation of HECT-type ubiquitin ligase Smurf1. Embo Journal, 2011,2675-2689.
[12] Liu P,Jenkins N A,Copeland N G.A highly efficient recombineering-based method for generating conditional knockout mutations.Genome Res,2003,13(3):476-484.
[13] Sedivy J M,Joyner A L.Gene Targeting.Oxford:Oxford University Press,1992.
[14] 赵玲,杜晓兰,苏楠,等. HIF-1α条件性基因敲除嵌合体小鼠的获得. 第三军医大学学报, 2007(14): 1361-1363. Zhao L,Du X L,Su N,et al.Generation of chineras for HIF-1αconditional knockout mice. Acta Academiae Medicinae Militaris Tertiae, 2007(14): 1361-1363.
[15] 冯贵莲,舒洋,王保曼,等, Tex101条件性基因敲除小鼠模型的建立和表型鉴定. 上海交通大学学报(医学版), 2014,1: 1-6. Feng G L,Shu Y,Wang B M,et al.Generation of Tex101 conditional knockout mice and phenotype identification.Journal of Shanghai Jiao Tong University (Medical Science),2014,1: 1-6.
[16] Schm D,Tsupprian M,Rajewsky K. Vagaries of conditional gene targeting.Nat Immunol,2007,8(7):665-668.
[17] Shiina H,Matsumoto T,Sato T,et al.Premature ovarian failure in androgen receptor-deficient mice. Proc Natl Acad Sci USA,2006,103(1):224-229.
[18] Rojek A,Fuchtbauer E M,Kwon T H,et al.Severe urinary concen-trating defect in renal collecting duct-selective AQP2 conditional-knockout mice.Proc Natl Acad Sci USA,2006,103(15):6037-6042.
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