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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2014, Vol. 34 Issue (12): 107-111    DOI: 10.13523/j.cb.20141215
综述     
基因捕获技术的现状及应用
王明科1,2,3, 孙慧勤2, 粟永萍2, 邹仲敏4
1. 中国人民解放军海军医学研究所 上海 200433;
2. 第三军医大学军事预防医学院全军复合伤研究所 创伤、烧伤与复合伤国家重点实验室 重庆 400038;
3. 解放军441医院 福鼎 355200;
4. 第三军医大学军事预防医学院毒理学研究所 重庆 400038
Current Status and Application of Gene Trapping
WANG Ming-ke1,2,3, SUN Hui-qin2, SU Yong-ping2, ZOU Zhong-min4
1. Naval Medical Research Institute, Shanghai 200433, China;
2. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China;
3. No. 441 Hospital of PLA, Fuding 355200, China;
4. Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
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摘要:

随着越来越多生物基因组测序的完成,生物医学研究已进入后基因组时代。基因捕获技术由于其在克隆、发现新基因及揭示基因功能方面具有独特的优点,已成为功能基因组时代研究的有力工具,在生物医学研究各个领域的应用日趋广泛。主要对基因捕获技术的原理、分类、一般操作流程、优缺点及基因捕获在发育生物学、新基因鉴定、干细胞分化、肿瘤和生殖医学研究方面的应用作一综述,为相关研究者提供借鉴。

关键词: 基因捕获发育生物学新基因鉴定干细胞分化肿瘤生殖医学    
Abstract:

With the completion of genome sequencing on more and more organisms, post-genomic era has begun. Gene trapping, as a powerful tool in the functional-genomic era, has been widespreadly applicated in many fields of biomedicine research because of its great merits in cloning, finding novel genes and unraveling their biological functions. The principles, classifications, manipulation procedures, advantages and disadvantages of gene trap technique and its application in developmental biology, novel gene identification, stem cells differentiation, cancer and reproductive medicine research are discussed, which will provide helpful references for relevant researchers.

Key words: Gene trapping    Developmental biology    Novel gene identification    Stem cells differentiation    Cancer    Reproductive medicine
收稿日期: 2014-10-08 出版日期: 2014-12-25
ZTFLH:  Q789  
基金资助:

国家自然科学基金(31201035)、国家"973"计划(2005CB522605)、全军医学科技"十二五"重大项目(AWS11C004)资助项目

通讯作者: 邹仲敏     E-mail: zouzhmin@yahoo.com
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引用本文:

王明科, 孙慧勤, 粟永萍, 邹仲敏. 基因捕获技术的现状及应用[J]. 中国生物工程杂志, 2014, 34(12): 107-111.

WANG Ming-ke, SUN Hui-qin, SU Yong-ping, ZOU Zhong-min. Current Status and Application of Gene Trapping. China Biotechnology, 2014, 34(12): 107-111.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20141215        https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I12/107


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