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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2014, Vol. 34 Issue (12): 91-96    DOI: 10.13523/j.cb.20141213
技术与方法     
木糖发酵生产高纯度D-乳酸大肠杆菌工程菌LHY02的构建
鲁泓鹰, 何虎, 刘枣, 王永泽, 王金华
工业发酵湖北省协同创新中心 发酵工程教育部重点实验室 湖北工业大学 武汉 430068
Engineering of an Escherichia coli Strain LHY02 for Production of Optically Pure D-lactic Acid from Xylose
LU Hong-ying, HE Hu, LIU Zao, WANG Yong-ze, WANG Jin-hua
Hubei Cooperative Innovation Center for Industrial Fermentation, Key Laboratory of Fermentation Engineering Ministry of Education, Hubei University of Technology, Wuhan 430068, China
 全文: PDF(639 KB)   HTML
摘要:

高效利用木糖发酵生产D-乳酸或其他生物质产品,是充分利用木质纤维素的一个关键问题。以高效利用木糖产L-乳酸的Escherichia coli WL204为出发菌株,采用RED基因置换技术将ldhL基因置换为ldhA基因,获得一株能利用木糖产D-乳酸的大肠杆菌工程菌株Escherichia coli LHY02,该菌株利用10%木糖发酵,D-乳酸产量达到84.4 g/L,产物光学纯度达到99.5%。此外,该菌株仍然具有较好的利用葡萄糖产D-乳酸的能力。

关键词: 木糖D-乳酸大肠杆菌工程菌RED基因置换技术    
Abstract:

Efficient utilization of xylose is an import microbial trait for fermentative production of bio-based products using renewable lignocellulosic feedstocks. Escherichia coli WL204 was previously engineered for fermentative production of optically pure L-lactic acid from xylose. E. coli WL204 was reengineered for D-lactic acid production by functionally replacing the l-lactate dehydrogenase gene (ldhL) with a D-lactate dehydrogenase (ldhA), resulting in strain E. coli LHY02. In 10% xylose fermentation test, LHY02 produced 84 g/L D-lactic acid with an optical purity of 99.5% and a volumetric productivity of 0.93 g/L/h.

Key words: Xylose    D-lactate    Engineered Escherichia coli    RED gene replacement techniques
收稿日期: 2014-10-08 出版日期: 2014-12-25
ZTFLH:  Q789  
基金资助:

国家自然科学基金(NSFC31070094)、湖北省自然科学基金(2011CDA008;2011CDB076)资助项目

通讯作者: 王永泽     E-mail: wangyongze@mail.hbut.edu.cn
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引用本文:

鲁泓鹰, 何虎, 刘枣, 王永泽, 王金华. 木糖发酵生产高纯度D-乳酸大肠杆菌工程菌LHY02的构建[J]. 中国生物工程杂志, 2014, 34(12): 91-96.

LU Hong-ying, HE Hu, LIU Zao, WANG Yong-ze, WANG Jin-hua. Engineering of an Escherichia coli Strain LHY02 for Production of Optically Pure D-lactic Acid from Xylose. China Biotechnology, 2014, 34(12): 91-96.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20141213        https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I12/91


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