技术与方法 |
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重组抗凝蛋白-新蛭素的原核表达研究 |
张超1, 巩蔚1, 郭莹莹1, 孙卫国2, 姚敏3, 于爱平1 |
1. 中国人民解放军军事医学科学院放射与辐射研究所 北京 100850;
2. 中国人民解放军第309医院 北京 100091;
3. 中国人民解放军医学图书馆 北京 100039 |
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The Prokaryotic Expression of an Anti-coagulant Protein of EH |
ZHANG Chao1, GONG Wei1, GUO Ying-ying1, SUN Wei-guo2, YAO Min3, YU Ai-ping1 |
1. Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, China;
2. The 309 hospital, PLA, Beijing 100091, China;
3. The Medical Library of the Chinese PLA, Beijing 100039, China |
引用本文:
张超, 巩蔚, 郭莹莹, 孙卫国, 姚敏, 于爱平. 重组抗凝蛋白-新蛭素的原核表达研究[J]. 中国生物工程杂志, 2014, 34(12): 69-77.
ZHANG Chao, GONG Wei, GUO Ying-ying, SUN Wei-guo, YAO Min, YU Ai-ping. The Prokaryotic Expression of an Anti-coagulant Protein of EH. China Biotechnology, 2014, 34(12): 69-77.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20141210
或
https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I12/69
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[1] Pineo G F, Hull R D.Hirudin and hirudin analogues as new anticoagulant agents. Curr Opin Hematol, 1995, 2(5): 380-385.
[2] Ruef Johannes, Katus Hugo A. New antithrombotic drugs on the horizon. Expert Opinion on Investigational Drugs, 2003, 12 (5): 781-797.
[3] Markwardt F. The development of hirudin as an antithrombotic drug. Thromb Res, 1994, 74(1): 1-23.
[4] Rewinkel J B, Adang A E.Strategies and progress towards the ideal orally active thrombin inhibitor. Current Pharmaceutical Design, 1999, 5(12): 1043-1075.
[5] Wallace A, Dennis S, Hofsteenge J, et al. Contribution of the N-terminal region of hirudin to its interaction with thrombin. Biochemistry, 1989,28(26):10079-10084.
[6] Wirsching F, Opitz T, Dietrich R, et al. Display of functional thrombin inhibitor hirudin on the surface of phage M13. Gene,1997, 204(1-2):177-184.
[7] Syed S, Schuyler P D, Kulczycky M, et al. Potent antithrombin activity and delayed clearance from the circulation characterize recombinant hirudin genetically fused to albumin. Blood,1997,89(9):3243-3252.
[8] McKenzie C R, Abendschein D R, Eisenberg P R. Sustained inhibition of whole-blood clot procoagulant activity by inhibition of thrombus-associated factor Xa. Arterioscler Thromb Vasc Biol,1996,16(10):1285-1291.
[9] 秦晓永,于爱平,王文文,等.抗凝蛋白EH体外活性检测条件的建立.中国生物工程杂志,2011,31 (5):108-112. Qin X Y,YU A P,Wang W W,et al.The antithrombin activity detection of EH in vitro.China Biotechnology,2011,31(5):108-112.
[10] Zhang Chuanling, Yu Aiping, Yuan Bin, et al. Construction and functional evaluation of hirudin derivatives with low bleeding risk. Thrombosis and Haemostasis, 2008, 99 (2): 324 -330.
[11] 王文文.抗凝蛋白的药效学及蛋白表达的优化.天津:天津大学,2012. Wang W W.Pharmacodynamic study of EH and optimization of its expression.Tianjin:Tientsin University,2012.
[12] 郝木强,李彦英,刘春杰, 等. 重组低出血抗凝蛋白在毕赤酵母中的中试发酵工艺研究及其纯化与鉴定.生物技术通讯,2013,(3):314-319. Hao M Q, Li Y Y, Liu C J,et al. Pilot-scale fermentation study of a low bleeding anticoagulant protein in Pichia pastoris.Letters in Biotechnology,2013,3:314-319.
[13] 国家药典委员会.中华人民共和国药典(三部).北京:中国医药科技出版社,2010.附录34-35. Chinese Pharmacopoeia Commission.Pharmacopoeia of the People's Republic of China(3 Volumes).Beijing:China Medical Science Press,2010:Appendi 34-35.
[14] Poet P, Scacheri E, Benatti L,et al. Production of the HV 1 variant of hirudin by recombinant DNA methodology. Blood Coagul Fibrinolysis, 1991,2(1):113120.
[15] Jizhong Yang, Xiangshan Zhou, Yuanxing Zhang. Improvement of recombinant hirudin production by controlling NH4+ concentration in Pichia pastoris fermentation. Biotechnology Letters, 2004,26(12):1013-1017.
[16] 龙铟,刘家云,刘莉, 等.水蛭素在毕赤酵母中的分泌表达.第四军医大学学报,2006,27 (8):673-676. Long Y,Liu J Y, Liu L,et al.Secretory expression of hirudins in Pichia pastoris.Journal of the Fourth Military Medical University,2006,27(8):673-676.
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