解淀粉芽孢杆菌KN-BL-1及其发酵豆粕产抗菌肽类物质的研究

doi:10.13523/j.cb.20141010

中国生物工程杂志

2014, Vol. 34

Issue (10): 61-66 DOI: 10.13523/j.cb.20141010

研究报告

解淀粉芽孢杆菌KN-BL-1及其发酵豆粕产抗菌肽类物质的研究

陈洁梅, 张灿辉, 艾田

暨南大学生命科学技术学院广州 510632

Study of the Antibacterial Peptides Produced by Bacillus amyloliquefaciens KN-BL-1 and Its Fermented Soybean Meal

CHEN Jie-mei, ZHANG Can-hui, AI Tian

College of Life Science and Technology, Jinan University, Guangzhou 510632, China

全文: PDF(958 KB) HTML

摘要：

以牛津杯法比较解淀粉芽孢杆菌KN-BL-1及其发酵豆粕的抑菌效果,通过酸沉淀法分离粗多肽,确定产生抑菌效果的为多肽类物质,通过DEAE-Sepharose离子交换层析及Sephadex G-25凝胶过滤层析分离纯化粗多肽以研究其各组分的抑菌效果,并使用4000 Q TRAP液相色谱-质谱联用仪初步确定抗菌肽样品的分子量范围.结果表明,KN-BL-1及其发酵豆粕浸提液对S.aureus等革兰氏阳性菌均有明显的抑菌效果;粗多肽经过层析分离纯化后,三个峰表现抑菌特性,其中有一个抑菌效果最好,抑菌圈清晰,直径为19.8 mm.

关键词： 发酵豆粕; 抗菌肽; 抑菌效果

Abstract:

The inhibitory effect of Bacillus amyloliquefaciens KN-BL-1 and its fermented soybean meal were compared by oxford cup method. The crude peptides which separated by acid-precipitation were determined as the effective antibacterial ingredients. In order to study the inhibitory effect of each component of the crude peptides, DEAE-Sepharose ion exchange chromatography and Sephadex G-25 gel filtration chromatography methods were used to separate the crude peptides and the molecular weight of the antibacterial peptides were initially identified by 4000 Q TRAP HPLC-MS. The results showed that the KN-BL-1 and its fermented soybean meal had a significant inhibitory effect to S.aureus and other Gram-positive bacteria. After separated and purified through chromatography, three elution peaks showed antibacterial properties.one of the three had the strongest inhibitory effect, and its diameter of antibiotic circle was 19.8 mm.

Key words: Fermented soybean meal Antimicrobial peptides Inhibitory effect

收稿日期: 2014-07-29 出版日期: 2014-10-25

ZTFLH:

Q819

基金资助:

广东省教育部产学研结合项目(2010B090400379)资助项目

通讯作者: 陈洁梅 E-mail: tchjm@jnu.edu.cn

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章

引用本文:

陈洁梅, 张灿辉, 艾田. 解淀粉芽孢杆菌KN-BL-1及其发酵豆粕产抗菌肽类物质的研究[J]. 中国生物工程杂志, 2014, 34(10): 61-66.

CHEN Jie-mei, ZHANG Can-hui, AI Tian. Study of the Antibacterial Peptides Produced by Bacillus amyloliquefaciens KN-BL-1 and Its Fermented Soybean Meal. China Biotechnology, 2014, 34(10): 61-66.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20141010 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I10/61

[1] Manzini M C, Perez K R, Riske K A, et al.Peptide: lipid ratio and membrane surface charge determine the mechanism of action of the antimicrobial peptide BP100.Conformational and functional studies.Biochimica et Biophysica Acta, 2014,1838(7):1985-1999.

[2] Bustillo M E, Fischer A L, LaBouyer M A, et al.Modular analysis of hipposin, a histone-derived antimicrobial peptideconsisting of membrane translocating and membranepermeabilizing fragments.Biochimica et Biophysica Acta,2014, 1838(9):2228-2233.

[3] Nguyen L T, Haney E F, Vogel H J. The expanding scope of antimicrobial peptidestructures and their modes of action.Trends Biotechnol, 2011,29(9):464-472.

[4] Harrison P L, Abdel-Rahman M A, Keith Miller, et al.Antimicrobial peptides from scorpion venoms.Toxicon, 2014, 88:115-137.

[5] Yoon J H, Ingale S L, Kim J S, et al.Effects of dietary supplementation with antimicrobial peptide-P5 on growth performance, apparent total tract digestibility, faecal and intestinal microflora and intestinal morphology of weanling pigs.Food Agric, 2013,93(3):587-592.

[6] Xiong X, Yang H S, Li L, et al.Effects of antimicrobial peptides in nursery diets on growth performance of pigs reared on five different farms.Livestock Science,2014, 167:206-210.

[7] Li Chun-Yi, Lu Jin-Jenn, Wu Chean-Ping, et al.Effects of probiotics and bremelain fermented soybean meal replacing fish meal on growth performance, nutrient retention and carcass traits of broilers.Livestock Science, 2014,163:94-101.

[8] Rist V T S, Weiss Eva, Sauer Nadja, et al.Effect of dietary protein supply originating from soybean meal or casein on the intestinal microbiota of piglets.Anaerobe, 2014,25:72-79.

[9] Ongena M, Jacques P. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology, 2008,16(3):115-125.

[10] Wu Shi-mei, Jia Shi-fang, Sun Dan-dan, et al.Purification and characterization of two novel antimicrobial peptides subpeptin JM4-A and subpeptin JM4-B produced by Bacillus subtilis JM4.Current Microbiology, 2005,51(5):292-296.

[11] 王东,孙力军,王雅玲,等.纳豆菌抗菌肽APNT-6对凡纳滨对虾的低温保鲜效果.水产学报, 2012,36(7):1133-1138. Wang D, Sun L J, Wang Y L, et al.Effect of antimicrobial peptide APNT-6 produced by Bacillus natto on fresh-keeping of Litopenaeus vannamei at low temperature.Journal of Fisheries of China, 2012,36(7):1133-1138.

[12] 马宗旺.海洋摩加夫芽孢杆菌 B0621A产生脂肽类活性化合物的研究.沈阳:中国科学院沈阳应用生态研究所,2012. Ma Z W.Isolation, structural identification and activity investigation of lipopeptides produced by a marine-derived Bacillus mojavensis B0621A.Shenyang:Institute of Applied Ecology, Chinese Academy of Sciences, 2012.

[1]	邓蕊,曾佳利,卢雪梅. 基于Musca domestica cecropin的抗肿瘤小分子衍生肽筛选及构效关系解析^*[J]. 中国生物工程杂志, 2021, 41(11): 14-22.
[2]	唐馨,毛新芳,马彬云,苟萍. 抗菌肽的研究现状和挑战 ^*[J]. 中国生物工程杂志, 2019, 39(8): 86-94.
[3]	杨隆兵,国果,马慧玲,李妍,赵欣宇,苏佩佩,张勇. 家蝇抗菌肽AMPs17蛋白原核表达条件的优化及其抗真菌活性检测 ^*[J]. 中国生物工程杂志, 2019, 39(4): 24-31.
[4]	唐健雪,肖永乐,彭俊杰,赵世纪,万小平,高荣. 融合抗菌肽基因在重组毕赤酵母的表达及体外活性研究 ^*[J]. 中国生物工程杂志, 2018, 38(6): 9-16.
[5]	杨静,贾如涵,李文慧,石大林,邵明洋,韩跃武. 抗菌肽改良设计及抗炎作用的研究进展[J]. 中国生物工程杂志, 2018, 38(1): 57-61.
[6]	温赛, 刘怀然, 韩煦, 李天, 邢旋. 综述人工合成型抗菌肽及其药学应用研究进展[J]. 中国生物工程杂志, 2016, 36(8): 89-98.
[7]	刘晓明, 姜宁, 张爱忠, 蔡鹏. 杂合抗菌肽在毕赤酵母中的表达及其活性测定[J]. 中国生物工程杂志, 2016, 36(2): 81-89.
[8]	巫春旭, 卢雪梅, 金小宝, 朱家勇. 天蚕素类抗菌肽分子设计研究进展[J]. 中国生物工程杂志, 2016, 36(2): 96-100.
[9]	武如娟, 张日俊. 杂合抗菌肽设计及生物学活性的研究进展[J]. 中国生物工程杂志, 2013, 33(9): 94-100.
[10]	陈宇婷, 王长海, 严秀文, 黎军胜. 抗菌肽的设计及其应用[J]. 中国生物工程杂志, 2013, 33(7): 97-102.
[11]	明飞平, 杨军, 朱进美, 邝哲师, 李华周, 夏枫耿, 叶明强, 王候光, 赵祥杰, 黄志丰, 蔡海明, 施巨清, 马苗鹏, 张玲华. 5’非翻译区序列改建提高抗菌肽PR39表达[J]. 中国生物工程杂志, 2013, 33(12): 86-91.
[12]	陈武, 黎定军, 丁彦, 张旭, 肖启明, 周清明. 病原微生物对抗菌肽抗性机制的研究进展[J]. 中国生物工程杂志, 2012, 32(05): 97-106.
[13]	舒梅, 许杨, 徐熙, 涂追. 两种水生动物抗菌肽的原核表达及活性分析[J]. 中国生物工程杂志, 2011, 31(02): 56-61.
[14]	李朴,文阳安,刘靳波,扬细媚,周金敬,涂植光. 抗菌肽Bactenecin7重组质粒构建及其在乳酸菌的分泌表达和活性鉴定[J]. 中国生物工程杂志, 2009, 29(01): 70-74.
[15]	邓超,王联结. 阳离子抗菌肽的研究进展[J]. 中国生物工程杂志, 2008, 28(6): 100-107.

Viewed

Full text

Abstract

Cited

Shared

Discussed