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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (06): 75-83    DOI: 10.13523/j.cb.20140611
综述     
纤维素酶在酿酒酵母中的表达研究
杨华军1, 邹少兰2, 刘成2, 马媛媛2, 马向霞3, 洪解放2
1. 天津大学化工学院 天津 300072;
2. 天津大学石化中心 天津 300072;
3. 天津市医药集团技术发展有限公司 天津 300193
Advance in Research on Cellulase Expression in Saccharomyces cerevisiae
YANG Hua-jun1, ZOU Shao-lan2, LIU Cheng2, MA Yuan-yuan2, MA Xiang-xia3, HONG Jie-fang2
1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2. Tianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China;
3. Tianjin Pharmaceutical Tech-Development Co., Ltd., Tianjin 300193, China
 全文: PDF(485 KB)   HTML
摘要：

统合生物加工过程（Consolidated bioprocessing，CBP）具有应用于纤维素乙醇生产的潜力，而该技术的关键是构建能有效降解纤维素的工程菌株。酿酒酵母是传统的乙醇发酵菌株，作为CBP宿主菌株具有很多优势，因此在酿酒酵母中表达纤维素酶引起研究者的普遍关注。综述了纤维素酶基因在酿酒酵母中表达的影响因素，包括基因表达盒表达元件（启动子、信号肽和终止子等）、纤维素酶基因拷贝数及存在形式以及纤维素酶基因来源等，并对一种和多种纤维素酶基因在酿酒酵母中的表达及构建得到的CBP菌株研究进展做了简要介绍。
关键词： 纤维素酶;  统合生物加工;  酿酒酵母;  纤维素乙醇    
Abstract:

Consolidated bioprocessing (CBP) is a promising technology for lignocellulosic ethanol production, and the key to CBP is the engineering of a microorganism that can efficiently utilize cellulose. Saccharomyces cerevisiae is a traditional ethanol producing strain, and it has many advantages as the CBP host strains, so the expression of cellulase in S. cerevisiae is causing great interest. The factors that influence the expression of cellulase genes in S. cerevisiae, including gene expression cassette expression elements (promoter, signal peptide and terminator, etc.), cellulase gene copy number and existing forms, the sources of cellulase gene and so on were reviewed. In addition, research progress about one or more cellulase genes expressed in S. cerevisiae and the construction of CBP strains were briefly introduced.
Key words: Cellulase    Consolidated bioprocessing(CBP)    Saccharomyces cerevisiae    Cellulosic ethanol
收稿日期: 2014-03-15 出版日期: 2014-06-25
ZTFLH:  Q786  
基金资助:

国家自然科学基金资助项目（30900033）
通讯作者: 洪解放     E-mail: hjf@tju.edu.cn
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引用本文:

杨华军, 邹少兰, 刘成, 马媛媛, 马向霞, 洪解放. 纤维素酶在酿酒酵母中的表达研究[J]. 中国生物工程杂志, 2014, 34(06): 75-83.

YANG Hua-jun, ZOU Shao-lan, LIU Cheng, MA Yuan-yuan, MA Xiang-xia, HONG Jie-fang. Advance in Research on Cellulase Expression in Saccharomyces cerevisiae. China Biotechnology, 2014, 34(06): 75-83.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140611    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I06/75


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