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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2021, Vol. 41 Issue (1): 62-71    DOI: 10.13523/j.cb.2011025
综述     
合成生物学改造微生物及生物被膜用于重金属污染检测与修复 *
常璐1,黄娇芳1,2,**(),董浩1,周斌辉3,朱小娟1,庄英萍1,**()
1 华东理工大学 生物反应器工程国家重点实验室 上海 200237
2 上海科技大学物质科学与技术学院 上海 201210
3 上海市生态环境局执法总队 上海 200235
A Review on Bioremediation and Detection of Heavy Metal Pollution by Synthetic Biological Engineered Microorganisms and Biofilms
CHANG Lu1,HUANG Jiao-fang1,2,**(),DONG Hao1,ZHOU Bin-hui3,ZHU Xiao-juan1,ZHUANG Ying-ping1,**()
1 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (ECUST), Shanghai 200237, China
2 School of Physical Science and Technology (SPST), Shanghai Tech University, Shanghai 201210, China
3 Shanghai Municipal Bureau of Ecology and Environment Law Enforcement Corps, Shanghai 200235, China
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摘要：

随着工业化进程不断加快,重金属污染日益加剧,尤其是水体的重金属污染,已严重威胁人类健康,迫切需要进行有效的污染修复。相比传统物理和化学修复,生物修复具有绿色环保和可持续性的特点。因为微生物生长繁殖迅速、生物被膜具有动态可调节和环境适应性好等特点,使其能更好耐受胁迫环境,在环境修复中有重要作用。合成生物学改造微生物及生物被膜用于环境污染生物修复近年兴起,成为未来重要的发展方向。主要概述了重金属污染的微生物修复机理和方法,结合可编程微生物被膜的最新研究成果,重点介绍了合成生物学改造微生物及生物被膜的分类与功能应用,以及在重金属铅、汞和镉等污染修复中的研究进展,讨论了重金属污染生物修复的发展方向。
关键词： 重金属污染;  生物修复;  合成生物学;  微生物;  生物被膜    
Abstract:

With the development of industrialization, heavy metal pollution has become a severe environmental hazard, in particular its pollution to water systems and consequently endangering human health, making its remediation an urgent issue. Compared with traditional physical and chemical remediation, bioremediation is green and sustainable. Microorganisms and microbial biofilms play an important role in bioremediation due to their rapid growth, intrinsic nature for dynamic adaption to environments and consequent tolerance to environmental stresses. Synthetic biology has provided a methodology for engineering and reprograming microorganisms and biofilms with robustness for more efficient degradation of environmental pollutants including heavy metals. This review summarizes the current status of heavy metal pollution, the bioremediation mechanism and research progress of microbial remediation, with a focus on the development of functional biofilms engineered by synthetic biology and their applications in bioremediation for heavy metal Pb, Hg, Cd and others. At the end, this review highlights the perspectives for bioremediation of heavy metal pollution.
Key words: Heavy metal pollution    Bioremediation    Synthetic biology    Microorganisms    Biofilms
收稿日期: 2020-11-12 出版日期: 2021-02-09
ZTFLH:  Q819  
基金资助: *上海市自然科学基金(19ZR1477100);国家自然科学基金(31872728);国家重点研发计划(2020YFA0908900)
通讯作者: 黄娇芳,庄英萍     E-mail: huangjf@ecust.edu.cn;ypzhuang@ecust.edu.cn
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引用本文:

常璐, 黄娇芳, 董浩, 周斌辉, 朱小娟, 庄英萍. 合成生物学改造微生物及生物被膜用于重金属污染检测与修复 *[J]. 中国生物工程杂志, 2021, 41(1): 62-71.

CHANG Lu, HUANG Jiao-fang, DONG Hao, ZHOU Bin-hui, ZHU Xiao-juan, ZHUANG Ying-ping. A Review on Bioremediation and Detection of Heavy Metal Pollution by Synthetic Biological Engineered Microorganisms and Biofilms. China Biotechnology, 2021, 41(1): 62-71.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2011025    或    https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I1/62

图1  重金属通过多种途径对人类产生有害影响
图2  合成生物学改造微生物在重金属污染检测和修复中的应用研究
图3  合成生物学改造的枯草芽孢杆菌生物被膜具有功能化[75]
图4  合成生物学改造微生物被膜在重金属污染修复中的研究进展
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