阿维拉霉素生物合成研究进展 <sup>*</sup>

doi:10.13523/j.cb.2009022

中国生物工程杂志

2021, Vol. 41

Issue (1): 94-102 DOI: 10.13523/j.cb.2009022

综述

阿维拉霉素生物合成研究进展 ^*

宇光海(

),彭海芬,王翱宇

河南工业大学生物工程学院郑州 450001

Research Progress of Avilamycin Biosynthesis

YU Guang-hai(

),PENG Hai-fen,WANG Ao-yu

College of Bioengineering, Henan University of Technology, Zhengzhou 450001, China

全文: PDF(22143 KB) HTML

摘要：

阿维拉霉素是由绿色产色链霉菌生产的寡糖类抗生素,对革兰氏阳性致病菌具有极强抗性,是一种新型饲料添加剂,广泛应用于肉鸡、仔猪等畜禽养殖中。以阿维拉霉素最新国内外研究进展为基础,综述了阿维拉霉素的抑菌机制、结构改造、菌种选育及发酵优化等方面的研究进展,重点阐述了阿维拉霉素的生物合成基因簇、合成途径及生物合成转录调控机制,探讨了提高阿维拉霉素产量的基因工程改造策略,为阿维拉霉素高效合成及工业化高产工程菌株构建等相关研究提供一定的理论参考。

关键词： 阿维拉霉素; 合成途径; 合成调控机制; 产量提高策略

Abstract:

Avilamycin, an oligosaccharide antibiotic with strong antibacterial effect to Gram-positive intestinal pathogenic bacteria, has been widely used in livestock and poultry breeding, such as broiler chickens and piglets, as a new type of feed additive. The antibacterial mechanism, structural modification, high yield strain breeding and fermentation optimization of avilamycin were briefly reviewed and the gene clusters, synthetic pathways and transcriptional regulation mechanisms of avilamycin were highlighted, based on research progress in the past five years, in this work. Further, the strategies of genetic engineering to improve the yield of avilamycin were discussed, which could provide some references for the efficient synthesis of avilamycin and the construction of industrial high-yielding engineered strains.

Key words: Avilamycin Synthetic pathway Synthesis regulation mechanism Strategies to increase production

收稿日期: 2020-09-11 出版日期: 2021-02-09

ZTFLH:

Q819

基金资助: * 国家自然科学基金(21506048)

通讯作者: 宇光海 E-mail: guanghaiyu66@haut.edu.cn

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引用本文:

宇光海, 彭海芬, 王翱宇. 阿维拉霉素生物合成研究进展 ^*[J]. 中国生物工程杂志, 2021, 41(1): 94-102.

YU Guang-hai, PENG Hai-fen, WANG Ao-yu. Research Progress of Avilamycin Biosynthesis. China Biotechnology, 2021, 41(1): 94-102.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2009022 或 https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I1/94

图1 阿维拉霉素分子结构示意图[15]

图2 阿维拉霉素的作用机制[18]

表1 阿维拉霉素合成基因簇中的主要功能基因

图3 阿维拉霉素生物合成基因簇[8]

图4 推测的阿维拉霉素A生物合成途径[8]

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