植物细胞器RNA编辑因子的功能及其作用机制 <sup>*</sup>

doi:10.13523/j.cb.1907029

中国生物工程杂志

2020, Vol. 40

Issue (3): 125-131 DOI: 10.13523/j.cb.1907029

综述

植物细胞器RNA编辑因子的功能及其作用机制 ^*

王柯茹,朱鸿亮(

)

中国农业大学食品科学与营养工程学院北京 100083

Functions of RNA Editing Factors and Its Mechanisms in Plant Organelles

WANG Ke-ru,ZHU Hong-liang(

)

College of Food Science and Nutrutuinal Engineering, China Agricultural University, Beijing 100083, China

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摘要：

在植物线粒体和叶绿体转录本上,数百个胞嘧啶(C)位点经脱氨基反应变为尿嘧啶(U),这是一种在转录本水平上对遗传信息进行修饰或调控的机制.在植物细胞器中,RNA编辑过程需要不同家族的RNA编辑因子相互作用组装成复杂的编辑复合体,特异地识别编辑位点进行编辑.最初的研究发现,植物RNA编辑受到高特异性五环肽重复(pentatricopeptide repeat, PPR)蛋白的调控,目前在植物中发现400多种PPR家族蛋白,编辑作用复杂.之后对RNA编辑因子互作蛋白/多细胞器RNA编辑因子(RNA editing factor interacting proteins /multiple organellar RNA editing factors,RIP/MORF),细胞器RNA识别基序(organelle RNA recognition motif,ORRM),细胞器锌指蛋白(organelle zinc-finger,OZ)等的研究表明,这些非PPR蛋白组分可以与PPR蛋白形成编辑复合体,共同参与编辑,且RNA编辑复合体具有多样性.RNA编辑因子的缺失会引起植物的生长发育受阻,果实成熟延迟等,对RNA编辑因子的研究显得尤为重要.对植物中RNA编辑因子的功能及其作用机制研究进展进行综述,旨在为后续RNA编辑的研究提供一定的参考.

关键词： RNA编辑; 细胞器; PPR蛋白; 非PPR蛋白

Abstract:

In flowering plants, cytosine C at hundreds of sites is usually converted into uracil U by deamination in mitochondrials and chloroplasts, which is a regulation mechanism of genetic information at the transcriptional level. RNA editing requires protein-protein or protein-RNA interaction to assemble into a complex"editosome" for recognition at special sites in plant organelles. Reachers have found that plant RNA editing is regulated by editosome pentapeptide repeat PPR proteins, and nor-PPR protein including that RNA editing factor interacting proteins/multiple organellar RNA editing factor (RIP/MORF), organelle RNA recognition motif(ORRM) proteins, organelle zinc-finger (OZ) proteins and others. These explain that plant RNA editing not only has a high diversity in PPR specific factors, but also nor-PPR protein components. The absence of RNA editing will cause adverse effects such as stunted growth, development of plants and fruit ripening. The most recent progress in the field and discuss the editosomes for the evolution of RNA editing and for futue findings were systematically summarized.

Key words: RNA editing Organelles PPR protein Nor-PPR protein

收稿日期: 2019-07-15 出版日期: 2020-04-18

ZTFLH:

Q819

基金资助: * 国家自然科学基金(31972472,31672208)

通讯作者: 朱鸿亮 E-mail: hlzhu@cau.edu.cn

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引用本文:

王柯茹,朱鸿亮. 植物细胞器RNA编辑因子的功能及其作用机制 ^*[J]. 中国生物工程杂志, 2020, 40(3): 125-131.

WANG Ke-ru,ZHU Hong-liang. Functions of RNA Editing Factors and Its Mechanisms in Plant Organelles. China Biotechnology, 2020, 40(3): 125-131.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.1907029 或 https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I3/125

表1 拟南芥中非PPR蛋白家族成员

图1 不同RNA编辑因子形成编辑复合体参与细胞器RNA编辑,RNA的特定位点发生C变U的编辑

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