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| Studying Mutiplex PCR system Consisted of Canine STR Loci PEZ1, PEZ5, PEZ20 |
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Abstract Abstract: Objective: It is applied to canine STR genotyping by studying the optimum amplification condition of mutiplex PCR system consisted of STR loci PEZ1, PEZ5 and PEZ20. Methods: Gaining the best concentration of the three primer pairs by Orthogonal design, then optimizing the concentration of Mg2+ and dNTP. The amplified products were detected by polyacrylamide gel electrophoresis and silver strains. Results: In condition that the concentration of three primer pairs PEZ1、PEZ5、PEZ20 which was 0.1μM、0.2μM、0.1μM severally, Mg2+ 3.0 mM and dNTP 0.2 mM , the objective strips of three STR loci are clear and similar intensity in this mutiplex PCR system, with faint and little of non-purpose strips. The mulplex system can applied to the canine STR genotyping test.
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Received: 13 March 2006
Published: 15 June 2006
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