ISOLATION OF A BACTERIUM WITH HIGH PRODUCT OF ALKALINE MN-SUPEROXIDE DISMUTASE , GENECLONE AND SEQUENCE ANALYSIS

China Biotechnology

2007, Vol. 27

Issue (5): 101-106 DOI:

ISOLATION OF A BACTERIUM WITH HIGH PRODUCT OF ALKALINE MN-SUPEROXIDE DISMUTASE , GENECLONE AND SEQUENCE ANALYSIS

Kan Guoshi

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Abstract

A high-product Superoxide dismutase(SOD) bacterial strain was first obtained from the soil around the warm spring of Changbai Mountain, which is alkaline-resistant. The nature analysis by the inhibition of H2O2 and chloroform-ethanol indicated that the SOD was Mn-SOD. It belongs to bacillus genus by the modal character, physiological and biochemical character. The phylogenetic analyses based on 16SrDNA sequence indicated that the strain 110-2 was closed to Bacillus sp.MO6 with 100% identity and 99% with Bacillus licheniformis. According to the sodA sequence of Bacillus licheniformis and conservative regions of many kinds of bacillus, which were published in GenBank, two pairs of primers were designed and the complete sequence of Mn-SOD(600bp) and the core fragment(430bp) were amplified by PCR techniques. The recombinant plasmid pMD18-SOD was built successfully.

Key words： Mn-Superoxide dismutase gene clone sequence analysis

Received: 11 September 2006 Published: 25 May 2007

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Cite this article:

Kan Guoshi . ISOLATION OF A BACTERIUM WITH HIGH PRODUCT OF ALKALINE MN-SUPEROXIDE DISMUTASE , GENECLONE AND SEQUENCE ANALYSIS. China Biotechnology, 2007, 27(5): 101-106.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I5/101

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