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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (09): 36-42    DOI: Q815
    
Effect of Complex and Synthetic Medium on Extracellular Production of α-Cyclodextrin Glycosyltransferase in E.coli
CHENG Jing1,2,WU Dan1,2,CHEN Sheng1,2,WU Jing1,2,CHEN Jian1,2
1.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2.School of Biotechnology, Jiangnan University,Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China  
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Abstract  

In order to achieve efficient extracellular production of α-cyclodextrin glycosyltransferase (α-CGTase) , E.coli BL21 (DE3) carrying the α-cgt gene of Paenibacillus macerans JFB05-01 was investivated. By comparison of different induction conditions in complex and synthetic medium, the highest extracellular α-CGTase activity reached 113.0 U/mL (hydrolysis activity was 79100.0 IU/mL）at 30 h of culture in synthetic medium. The optimized condition is as follows: fed 0.8 g/L/h lactose as inducer in synthetic medium, which contains glycine. The enzyme production from this condition was as 2.3 times as that in complex medium.

Key words： Paenibacillus macerans      α-Cyclodextrin glycosyltransferase      Extracellular production      E.coliSynthetic medium     
Received: 12 May 2010      Published: 25 August 2010
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CHENG Jing
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Cite this article:

CHENG Jing, TUN Dan, CHEN Cheng, TUN Jing, CHEN Jian. Effect of Complex and Synthetic Medium on Extracellular Production of α-Cyclodextrin Glycosyltransferase in E.coli. China Biotechnology, 2010, 30(09): 36-42.

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https://manu60.magtech.com.cn/biotech/Q815     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I09/36

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