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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (01): 65-69    DOI:
    
Studies on Detection of Salmonella typhimurium in Food by Multiplex Polymerase Chain Methods
FENG Fei1, XIE Zhen-wen2, ZENG Mu-heng1
1. College of Life Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. College of Agriculture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
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Abstract  

Four sets of primers were designed according to 16S rRNA, spvC, invB, fimA genes of Salmonella typhimurium, which were used to amplify genomic DNA of Salmonella and non Salmonellas by multiplex polymerase chain reaction (m-PCR). Specificity results showed that only Salmonella strains had specifically amplified the target fragments, but none others. Sensitivity of m-PCR assay for pure cell cultures of Salmonella typhimurium was 6.3×102cfu/ml. However the detection limits of this method for artificially contaminated cooked chicken, milk powder, and beef were 17cfu/g, 11cfu/g, 13.6cfu/g following 8h of enrichment, respectively. This multiplex PCR assay successfully identifed main virulence genes of Salmonella typhimurium and may be applied to rapid and sensitive detection of Salmonella typhimurium in food when combined with an enrichement step.



Key wordsSalmonella      Multiplex polymerase chain reaction      Rapid detection     
Received: 09 September 2010      Published: 25 January 2011
ZTFLH:  Q7  
Cite this article:

FENG Fei, XIE Zhen-wen, ZENG Mu-heng. Studies on Detection of Salmonella typhimurium in Food by Multiplex Polymerase Chain Methods. China Biotechnology, 2011, 31(01): 65-69.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I01/65


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