中国生物工程杂志

Aspergillus oryzae is an important microorganism, which was widely used in the fields of food, brewing, pharmacy and fermentation industry etc and was termed as a generally regarded as safe(GRAS) organism by the United States Food and Drug Administration (FDA).The strategies for promoting the expression of homologous and heterologous proteins in Aspergillus oryzae were reviewed. Which included the usage of strong promoters, multicopies of coding genes, optimization of culture medium and overexpression of the hemoglobin domains (HBD) etc. Heterologous proteins were usually exhibited low expression in Aspergillus oryzae due to the degradation by the proteinases from host. Therefore, development of proteinase auxotrophic stains of Aspergillus oryzae as host is neccessary. In addition, fusion of the heterologous protein with a highly secreted protein of Aspergillus oryzae was an alternative way to prompt the expression of heterologous protein in Aspergillus oryzae.