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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (03): 41-46    DOI:
    
Preparation and Biological Activity of Poly (γ-glutamic acid)-D-galactose- Cisplatin Complex Compound
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Abstract  

In order to express and purify the mono-phosphorylated PZR proteins, the bi-phosphorylated-site His-PZR recombined prokaryotic expressions plasmids was used as the template, then through the overlapping PCR the mono- phosphorylated-site PZR-Phe263(TTT) and PZR-Phe241(TTT) mutants were amplified. After recombined and purified, the mutants' and C-Src recombined prokaryotic expressions plasmids were co-transformed into the E.Coli. BL21. Through IPTG induction and Ni-NTA purification, the mono-phosphorylated PZR a and PZR b proteins were obtained successfully. These results laid a foundation for future study on the PZR bio-function mechanism.



Key wordsTargeted drug delivery;Poly(γ-glutamic acid);Cisplatin;Toxicity;Antitumor efficiency     
Received: 10 October 2008      Published: 31 March 2009
Cite this article:

CAO Xiao-Gong- Yan-Le- Wang-Chun-Ling- Jiao-Run-Zhi- Lu-Mei-Fang. Preparation and Biological Activity of Poly (γ-glutamic acid)-D-galactose- Cisplatin Complex Compound. China Biotechnology, 2009, 29(03): 41-46.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/41

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