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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (03): 74-79    DOI:
    
Construction of Conditional Target-trapping Vector
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Abstract  

Gene trapping is a powerful method for gene analysis relying on mutation generated by random insertion. Various of new kinds trapping vectors have been endlessly designed and applied for trapping because of the local of conventional trapping vector. Based on plasmid PL-451,combinding with the site specific recombination target sites—Loxp,Frt and LoxP511A,LoxP511B which mutant one base of the LoxP,we constructed a conditional gene trapping vector.The trapping vector contains multicloning sites for cloning of the homology regions,which make it can realize the target trapping depending on homologous recombination. Furthermore, the exprssion of promoterless EGFP with splice acceptor(SA)and polyA in the vector can tagging the work of the trapping cassettes.Construction of this vector establish substantial foundation for conditional trapping of target gene.



Key wordsgene trapping;mutant LoxP;conditional gene trapping vector;target trapping     
Received: 08 October 2008      Published: 31 March 2009
Corresponding Authors: Lin Wei   
Cite this article:

YANG Jian-Ling- Yang-Yi-Gong- Tao-Zhi-Yan- Wei-Lin. Construction of Conditional Target-trapping Vector. China Biotechnology, 2009, 29(03): 74-79.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/74

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