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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (03): 57-62    DOI:
    
Effects of Carbon Sources on Succinate Production by Dual-phase Fermentations of Recombinant Escherichia coli
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Abstract  

The effects of different carbon sources added to aerobic medium on cell growth, enzyme activity and metabolite distribution was investigated in dual-phase fermentations. The results showed that both cell density and enzyme activity were enhanced by adding 4mmol/L glucose or 12,54,80mmol/L sodium acetate to aerobic medium, respectively. Then centrifugated cells that had grown aerobically in different conditions were transferred to anaerobic fermentation, the enzyme activity and metabolite distribution changed. To analyze the anaerobic enzyme activity and metabolite distribution, it was concluded that during the anaerobic fermentation of Escherichia coli NZN111 overexpressed malic enzyme, PEP carboxykinase(PCK) was the key enzyme of succinate production, pyruvate kinase(PYK) was associated to the accumulation of byproduct pyruvate, and isocitrate lyase(ICL) have some influence on the concentration of succinate. The yield of succinate in anaerobic stage could reach 89.0%, which was 16.6% higher than that of the control by adding 80mmol/L sodium acetate as carbon source in aerobic medium.



Received: 31 October 2008      Published: 31 March 2009
ZTFLH:  Q815  
Cite this article:

WANG Yi-Na- Ma-Jiang-Feng- Chen-Ge-Quan- Zuo-Feng- Tun-Xiao-Hua- Jiang-Min. Effects of Carbon Sources on Succinate Production by Dual-phase Fermentations of Recombinant Escherichia coli. China Biotechnology, 2009, 29(03): 57-62.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I03/57

[1] Zeikus J G, Jain M K, Elankovan P. Biotechnology of succinic acid production and markets for derived industrial products. Appl Microbiol Biotechnol, 1999, 51: 545~552 [2] Willke T, Vorlo PK. Industrial bioconversion of renewable resources as an alternative to conventional chemistry. Appl Microbiol Biotechnol, 2004, 66:131~142 [3] Chatterjee R ,Millard C S ,Champion K, et al. Mutation of the ptsG gene results in increased production of succinate in fermentation of glucose by Escherichia coli. Appl Environ Microbiol, 2001, 67:148~154 [4] Vemuri G N, Eiteman M A, Altman E. Succinate production in dualphase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions. J Ind Microbiol Biotechnol, 2002, 28:325~332 [5] Andersson C, David H, Berglund K A, et al. Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli. Biotechnol Pr, 2007,23 (2):381~388 [6] 谢鑫,陈可泉,刘忠敏,等. 产丁二酸工程菌的构建及其厌氧发酵.生物工程学报,2008,24(1):101~105 Xie X, Chen K Q, Liu Z M, et al.Chin J Biotech, 2008, 24(1): 101~105 [7] Hong S H, Lee S Y. Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity. Biotechnology and Bioengineering, 2001, 74:89~95 [8] Hong S H, Lee S Y. Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli. Appl Microbiol Biotechnol, 2002, 58:286~290 [9] Van der Werf M J, Guettler M V, Jain M K, et al. Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z. Arch Microbiol, 1997,168:332~342 [10] Wu H, Li Z M, Zhou L,et al.Improved succinic acid production in anaerobic culture of a pflB ldhA double mutant of Escherichia coli by enhancing the anaplerotic activities in preceding aerobic culture. Appl Environ Microbiol. 2007,73(24): 7837~7843 [11] 陈可泉,韦萍,蔡婷,等. 反相高效液相色谱在发酵制备琥珀酸中的应用.生物加工过程,2005, 3(2): 50~52 Chen K Q, Wei P, Cai T, et al.Chinese Journal of Bioprocess Engineering, 2005,3(2):50~52 [12] Roe A J, Mclaggan D, Davidson I. Perturbation of anion balance during inhibition of growth of Escherichia coli by weak acid. Journa of Bacteriology, 1998, 180(4):767~772 [13] 姜岷,谢鑫,徐琳,等.过量表达苹果酸酶对E.coli FMJ39厌氧混合酸发酵的影响.中国生物工程杂志,2007, 27(1): 69~74 Jiang M, Xie X, Xu L, et al.China Biotechnology, 2007, 27(1):69~74 [14] Lee PC, Lee W G, Kwon S, et al. Succinic acid production by Anaerobiospirillum succiniciproducens:effects of the H2/CO2 supply and glucose concentration. Enzyme Microb Technol. 1999,24:549~554 [15] Lin H, Bennett G N, San K Y. Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield. Metab Eng, 2005, 7: 116~227 [16] Podkovyrov S M, Zeikus J G. Purification and characterization of phosphoenolpyruvate carboxykinase, catabolic CO2fixing enzyme, from Anaerobiospirillum succiniciproducen. Journal of General Microbiology, 1993,139:223~228

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