Advance in Fed-Batch Mammalian Cell Culture

China Biotechnology

2008, Vol. 28

Issue (11): 104-109 DOI:

Advance in Fed-Batch Mammalian Cell Culture

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Abstract

Fed-batch culture is the predominant mode of the current animal cell culture process for many recombinant protein production. Fed-batch culture operation is mainly based on the nutrient continuous consumption and demand of cells to design continuous or semi-continuous concentration feed medium to maintain or support high-density cell growth and improve volumetric productivity of target protein in the reactor. The main methods to improve production efficiency of fed-batch cells culture include the optimization of medium design, selection and optimization of feed strategy and regulation of cell metabolism.

Key words： Mammalian cells Fed-Batch Culture medium Control Strategy

Received: 26 June 2008 Published: 20 April 2009

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	LIU Xin-Mao
	CHEN Zhao-Lie

Cite this article:

. Advance in Fed-Batch Mammalian Cell Culture. China Biotechnology, 2008, 28(11): 104-109.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I11/104

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