中国生物工程杂志

【Objective】Prepare monoclonal antibody(MAb) using SP2/0 cell isolated from tumor in live mouse, and compare the efficiency of cell fusion with conventional method.【Methods】The SP2/0 cell were hypodermic injected in the back of the SPF level BALB/c mouse which were 8 week old. The SP2/0 cells were re-prepared by isolation from the tumor when it reached 2~3cm diameter. The fusion efficiency of SP2/0 cell from live tumor and from cell culture was compared by fusing with spleen cells. The relative affinity of MAbs made by two methods were also compared. 【Results】We have made six fusions. The fusion rate is 70.4% using SP2/0 cell from live tumor, while that is 44.6% using SP2/0 cell from cell culture. The relative affinity of MAbs made by two method were all above 1:100000. 【Conclusion】 We can significantly improve the fusion efficiency by fusion spleen cell with SP2/0 cell from live tumor.