中国生物工程杂志

Endo-type chitosanase (ChiN) was isolated from the chitosanase fermentation broth by membrane separation techniques and the conditions that oligo-chitosan was prepared by ChiN were also optimized. The number average molecular weight of prepared oligo-chitosan is about 1200. Analysis by the methods of TLC and HPLC, no monosaccharide exists in the enzymatic product. So we confirm that the major enzymatic products are oligo-chitosan. Oligo-chitosan can be produced rapidly and inexpensively by enzyme-catalyzed reaction which has laid a basis for the large-scare preparation of oligo-chitosan.