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Fish Gelatin Modification Using Prokaryotic Expression and Cell Surface Display Proline Hydroxylases |
HUANG Ming-zhu1,2,3,SHEN Qi-chang2,3,QIN Chun-yan2,3,XU Yang3,WEI Yi-ran3,CHEN Xue-lan1,2,3,*() |
1 National R&D Center of Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China 2 School of Life Science, Jiangxi Normal University, Nanchang 330022, China 3 School of Health, Jiangxi Normal University, Nanchang 330022, China |
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Abstract The poor gel properties of fish gelatin limit its commercial application. Enzyme-catalyzed modification of fish gelatin has great advantages such as environmental friendliness, safety and high efficiency. However, there are few gelatin-modified enzymes reported at present, and most of them are covalent cross-linking enzymes, which easily make gelatin form thermal irreversible gel. In this study, two proline hydroxylases that increase the non-covalent action of collagen were cloned and expressed in prokaryotic cells. After purification, the fish gelatin was catalyzed respectively using two proline hydroxylases. The results show that the two enzymes have the effect of improving the gel strength and texture characteristics of fish gelatin. In addition, the surface display technology of Corynebacterium crenatum was studied. The two enzymes were displayed on the surface of C. crenatum, and an immobilized enzyme system with C. crenatum as the carrier was prepared for studying the modification effect of two immobilized enzyme systems on fish gelatin. This study has enriched the catalytic enzyme system of fish gel and provided a new idea for the catalytic modification of fish gelatin.
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Received: 19 May 2023
Published: 01 December 2023
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Corresponding Authors:
*Xue-lan CHEN
E-mail: xuelanchen162@163.com
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